Unravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa.
supporting information to Krueger ...
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractTranscriptional regulation has a central role in cellular adaptation processes and is well investigated. In contrast, the importance of the post-transcriptional regulation on these processes is less well defined. The technological advancements have been critical to precisely quantify protein and mRNA level changes and hold promise to provide more insights into how post-transcriptional regulation determines phenotypes. In Pseudomonas aeruginosa the methyltransferase PrmC methylates peptide chain release factors to facilitate translation termination. Loss of PrmC activity abolishes anaerobic growth and leads to reduced production of quorum sensing-associated virulence factors. Here, by applying SILAC technology in combination with mRNA-sequencing, they provide evidence that the P. aeruginosa phenotype can be attributed to a change in protein to mRNA ratios of selected protein groups. The UAG-dependent translation termination was more dependent on PrmC activity than the UAA- and UGA-dependent translation termination. Additionally, a bias toward UAG stop codons in global transcriptional regulators was found. The finding that this bias in stop codon usage determines the P. aeruginosa phenotype is unexpected and adds complexity to regulatory circuits. Via modulation of PrmC activity the bacterial cell can cross-regulate targets independently of transcriptional signals, a process with an underestimated impact on the bacterial phenotype.
CitationUnravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa. 2016, 18 (10):3583-3592 Environ. Microbiol.
AffiliationTwincore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen Str. 7, 30625 Hannover, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- The peptide chain release factor methyltransferase PrmC is essential for pathogenicity and environmental adaptation of Pseudomonas aeruginosa PA14.
- Authors: Pustelny C, Brouwer S, Müsken M, Bielecka A, Dötsch A, Nimtz M, Häussler S
- Issue date: 2013 Feb
- Methylation of bacterial release factors RF1 and RF2 is required for normal translation termination in vivo.
- Authors: Mora L, Heurgué-Hamard V, de Zamaroczy M, Kervestin S, Buckingham RH
- Issue date: 2007 Dec 7
- Molecular basis for bacterial class I release factor methylation by PrmC.
- Authors: Graille M, Heurgué-Hamard V, Champ S, Mora L, Scrima N, Ulryck N, van Tilbeurgh H, Buckingham RH
- Issue date: 2005 Dec 22
- Coevolution between Stop Codon Usage and Release Factors in Bacterial Species.
- Authors: Wei Y, Wang J, Xia X
- Issue date: 2016 Sep
- Role of ribosome release in regulation of tna operon expression in Escherichia coli.
- Authors: Konan KV, Yanofsky C
- Issue date: 1999 Mar