Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.
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Authors
Fazli, MustafaRybtke, Morten
Steiner, Elisabeth
Weidel, Elisabeth
Berthelsen, Jens
Groizeleau, Julie
Bin, Wu
Zhi, Boo Zhao
Yaming, Zhang
Kaever, Volkhard
Givskov, Michael
Hartmann, Rolf W.
Eberl, Leo
Tolker-Nielsen, Tim

Issue Date
2017-04-16
Metadata
Show full item recordAbstract
Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.Citation
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. 2017 MicrobiologyopenAffiliation
Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.Journal
MicrobiologyOpenDOI
10.1002/mbo3.480PubMed ID
28419759Type
ArticleLanguage
enISSN
2045-8827ae974a485f413a2113503eed53cd6c53
10.1002/mbo3.480
Scopus Count
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/