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dc.contributor.authorFazli, Mustafa
dc.contributor.authorRybtke, Morten
dc.contributor.authorSteiner, Elisabeth
dc.contributor.authorWeidel, Elisabeth
dc.contributor.authorBerthelsen, Jens
dc.contributor.authorGroizeleau, Julie
dc.contributor.authorBin, Wu
dc.contributor.authorZhi, Boo Zhao
dc.contributor.authorYaming, Zhang
dc.contributor.authorKaever, Volkhard
dc.contributor.authorGivskov, Michael
dc.contributor.authorHartmann, Rolf W.
dc.contributor.authorEberl, Leo
dc.contributor.authorTolker-Nielsen, Tim
dc.date.accessioned2017-05-12T14:47:05Z
dc.date.available2017-05-12T14:47:05Z
dc.date.issued2017-04-16
dc.identifier.citationRegulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. 2017 Microbiologyopenen
dc.identifier.issn2045-8827
dc.identifier.pmid28419759
dc.identifier.doi10.1002/mbo3.480
dc.identifier.urihttp://hdl.handle.net/10033/620917
dc.description.abstractKnowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleRegulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalMicrobiologyOpenen
refterms.dateFOA2018-06-12T23:13:31Z
html.description.abstractKnowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.


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