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dc.contributor.authorAkmatov, Manas K
dc.contributor.authorKoch, Nadine
dc.contributor.authorVital, Marius
dc.contributor.authorAhrens, Wolfgang
dc.contributor.authorFlesch-Janys, Dieter
dc.contributor.authorFricke, Julia
dc.contributor.authorGatzemeier, Anja
dc.contributor.authorGreiser, Halina
dc.contributor.authorGünther, Kathrin
dc.contributor.authorIllig, Thomas
dc.contributor.authorKaaks, Rudolf
dc.contributor.authorKrone, Bastian
dc.contributor.authorKühn, Andrea
dc.contributor.authorLinseisen, Jakob
dc.contributor.authorMeisinger, Christine
dc.contributor.authorMichels, Karin
dc.contributor.authorMoebus, Susanne
dc.contributor.authorNieters, Alexandra
dc.contributor.authorObi, Nadia
dc.contributor.authorSchultze, Anja
dc.contributor.authorSix-Merker, Julia
dc.contributor.authorPieper, Dietmar H
dc.contributor.authorPessler, Frank
dc.date.accessioned2017-05-17T12:45:45Z
dc.date.available2017-05-17T12:45:45Z
dc.date.issued2017-05-12
dc.identifier.citationDetermination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort. 2017, 7 (1):1855 Sci Repen
dc.identifier.issn2045-2322
dc.identifier.pmid28500287
dc.identifier.doi10.1038/s41598-017-01212-6
dc.identifier.urihttp://hdl.handle.net/10033/620922
dc.description.abstractWe examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants' satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1-V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDetermination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort.en
dc.typeArticleen
dc.contributor.departmentTWINCORE; Zentrum für experimentelle und klinische Infectionsforsching GmbH, Feodor-Lynen Str. 17, 30625 Hannover, Germany.en
dc.identifier.journalScientific reportsen
refterms.dateFOA2018-06-13T00:03:37Z
html.description.abstractWe examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants' satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1-V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.


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