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dc.contributor.authorSomplatzki, Stefan
dc.contributor.authorMühlenhoff, Martina
dc.contributor.authorKröger, Andrea
dc.contributor.authorGerardy-Schahn, Rita
dc.contributor.authorBöldicke, Thomas
dc.date.accessioned2017-05-24T12:59:17Z
dc.date.available2017-05-24T12:59:17Z
dc.date.issued2017-05-12
dc.identifier.citationIntrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells. 2017, 17 (1):42 BMC Biotechnol.en
dc.identifier.issn1472-6750
dc.identifier.pmid28499450
dc.identifier.doi10.1186/s12896-017-0360-7
dc.identifier.urihttp://hdl.handle.net/10033/620928
dc.description.abstractPolysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown. To inhibit the function of ST8SiaII and ST8SiaIV specific ER IBs were generated starting from two corresponding hybridoma clones. Both IBs αST8SiaII-IB and αST8SiaIV-IB were constructed in the scFv format and their functions characterized in vitro and in vivo.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIntrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centr for infection researchen
dc.identifier.journalBMC biotechnologyen
refterms.dateFOA2018-06-13T00:43:55Z
html.description.abstractPolysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown. To inhibit the function of ST8SiaII and ST8SiaIV specific ER IBs were generated starting from two corresponding hybridoma clones. Both IBs αST8SiaII-IB and αST8SiaIV-IB were constructed in the scFv format and their functions characterized in vitro and in vivo.


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