Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA.
Name:
Supplemental Materials.pdf
Size:
2.083Mb
Format:
PDF
Description:
supplemental material
Name:
fig1_color.pptx
Size:
1.754Mb
Format:
Microsoft PowerPoint 2007
Description:
figure 1
Name:
fig4_color.pptx
Size:
617.6Kb
Format:
Microsoft PowerPoint 2007
Description:
figure 4
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Koshla, OksanaLopatniuk, Maria
Rokytskyy, Ihor
Yushchuk, Oleksandr
Dacyuk, Yuriy
Fedorenko, Victor
Luzhetskyy, Andriy N
Ostash, Bohdan
Issue Date
2017-05-20
Metadata
Show full item recordAbstract
Streptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNA(Leu)UAA, the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media. In addition, bldA mutant of J1074 was unable to produce endogenous antibacterial compounds and two heterologous antibiotics, moenomycin and aranciamycin, whose biosynthesis is directed by TTA-containing genes. We have employed a new TTA codon-specific β-galactosidase reporter system to provide genetic evidence that J1074 bldA mutant is impaired in translation of TTA. In addition, we have discussed the possible reasons for differences in the phenotypes of bldA mutants described here and in previous studies, providing knowledge to study bldA-based regulation of antibiotic biosynthesis.Citation
Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA. 2017 Arch. Microbiol.Affiliation
Helmholtz Institut für phsarmazeutische Forschung Saarland (HIPS), Universitätscampus E8.1, 66123 Saarbrücken, Germany.Journal
Archives of microbiologyPubMed ID
28528473Type
ArticleLanguage
enISSN
1432-072Xae974a485f413a2113503eed53cd6c53
10.1007/s00203-017-1389-7
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/