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    SubjectsProQ (2)RNA-seq (2)acid sphingomyelinase (1)bacterial pathogen (1)Cas9 (1)View MoreJournalMolecular cell (2)BIOspektrum (1)mBio (1)Nature Reviews Microbiology (1)Nucleic acids research (1)View MoreAuthorsVogel, Jörg (17)
    Vogel, Jörg (17)
    Förstner, Konrad U (4)Saliba, Antoine-Emmanuel (4)Westermann, Alexander J (3)View MoreYear (Issue Date)2018 (9)2017 (7)2019 (1)TypesArticle (17)

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    Now showing items 11-17 of 17

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    Global snapshots of bacterial RNA networks.

    Hör, Jens; Vogel, Jörg (2017-02-01)
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    The Major RNA-Binding Protein ProQ Impacts Virulence Gene Expression in Salmonella enterica Serovar Typhimurium.

    Westermann, Alexander J; Venturini, Elisa; Sellin, Mikael E; Förstner, Konrad U; Hardt, Wolf-Dietrich; Vogel, Jörg (Amercan Society of Microbiology, 2019-01-02)
    FinO domain proteins such as ProQ of the model pathogen
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    In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.

    Chao, Yanjie; Li, Lei; Girodat, Dylan; Förstner, Konrad U; Said, Nelly; Corcoran, Colin; Śmiga, Michał; Papenfort, Kai; Reinhardt, Richard; Wieden, Hans-Joachim; et al. (2017-01-05)
    Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.
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    ANNOgesic: a Swiss army knife for the RNA-seq based annotation of bacterial/archaeal genomes.

    Yu, Sung-Huan; Vogel, Jörg; Förstner, Konrad U (2018-09-01)
    To understand the gene regulation of an organism of interest, a comprehensive genome annotation is essential. While some features, such as coding sequences, can be computationally predicted with high accuracy based purely on the genomic sequence, others, such as promoter elements or noncoding RNAs, are harder to detect. RNA sequencing (RNA-seq) has proven to be an efficient method to identify these genomic features and to improve genome annotations. However, processing and integrating RNA-seq data in order to generate high-resolution annotations is challenging, time consuming, and requires numerous steps. We have constructed a powerful and modular tool called ANNOgesic that provides the required analyses and simplifies RNA-seq-based bacterial and archaeal genome annotation. It can integrate data from conventional RNA-seq and differential RNA-seq and predicts and annotates numerous features, including small noncoding RNAs, with high precision. The software is available under an open source license (ISCL) at https://pypi.org/project/ANNOgesic/.
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    Salmonella persisters undermine host immune defenses during antibiotic treatment.

    Stapels, Daphne A C; Hill, Peter W S; Westermann, Alexander J; Fisher, Robert A; Thurston, Teresa L; Saliba, Antoine-Emmanuel; Blommestein, Isabelle; Vogel, Jörg; Helaine, Sophie (American Association for the Advancement of Science, 2018-12-07)
    Many bacterial infections are hard to treat and tend to relapse, possibly due to the presence of antibiotic-tolerant persisters. In vitro, persister cells appear to be dormant. After uptake of Salmonella species by macrophages, nongrowing persisters also occur, but their physiological state is poorly understood. In this work, we show that Salmonella persisters arising during macrophage infection maintain a metabolically active state. Persisters reprogram macrophages by means of effectors secreted by the Salmonella pathogenicity island 2 type 3 secretion system. These effectors dampened proinflammatory innate immune responses and induced anti-inflammatory macrophage polarization. Such reprogramming allowed nongrowing Salmonella cells to survive for extended periods in their host. Persisters undermining host immune defenses might confer an advantage to the pathogen during relapse once antibiotic pressure is relieved.
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    RNA-binding proteins in bacteria.

    Holmqvist, Erik; Vogel, Jörg (Springer Nature, 2018-10-01)
    RNA-binding proteins (RBPs) are central to most if not all cellular processes, dictating the fate of virtually all RNA molecules in the cell. Starting with pioneering work on ribosomal proteins, studies of bacterial RBPs have paved the way for molecular studies of RNA-protein interactions. Work over the years has identified major RBPs that act on cellular transcripts at the various stages of bacterial gene expression and that enable their integration into post-transcriptional networks that also comprise small non-coding RNAs. Bacterial RBP research has now entered a new era in which RNA sequencing-based methods permit mapping of RBP activity in a truly global manner in vivo. Moreover, the soaring interest in understudied members of host-associated microbiota and environmental communities is likely to unveil new RBPs and to greatly expand our knowledge of RNA-protein interactions in bacteria.
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    CRP-cAMP mediates silencing of Salmonella virulence at the post-transcriptional level.

    El Mouali, Youssef; Gaviria-Cantin, Tania; Sánchez-Romero, María Antonia; Gibert, Marta; Westermann, Alexander J; Vogel, Jörg; Balsalobre, Carlos (2018-01-01)
    Invasion of epithelial cells by Salmonella enterica requires expression of genes located in the pathogenicity island I (SPI-1). The expression of SPI-1 genes is very tightly regulated and activated only under specific conditions. Most studies have focused on the regulatory pathways that induce SPI-1 expression. Here, we describe a new regulatory circuit involving CRP-cAMP, a widely established metabolic regulator, in silencing of SPI-1 genes under non-permissive conditions. In CRP-cAMP-deficient strains we detected a strong upregulation of SPI-1 genes in the mid-logarithmic growth phase. Genetic analyses revealed that CRP-cAMP modulates the level of HilD, the master regulator of Salmonella invasion. This regulation occurs at the post-transcriptional level and requires the presence of a newly identified regulatory motif within the hilD 3'UTR. We further demonstrate that in Salmonella the Hfq-dependent sRNA Spot 42 is under the transcriptional repression of CRP-cAMP and, when this transcriptional repression is relieved, Spot 42 exerts a positive effect on hilD expression. In vivo and in vitro assays indicate that Spot 42 targets, through its unstructured region III, the 3'UTR of the hilD transcript. Together, our results highlight the biological relevance of the hilD 3'UTR as a hub for post-transcriptional control of Salmonella invasion gene expression.
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