Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase.

Muruganandam, Gopinath; Raasakka, Arne; Myllykoski, Matti; Kursula, Inari; Kursula, Petri

dc.contributor.author	Muruganandam, Gopinath
dc.contributor.author	Raasakka, Arne
dc.contributor.author	Myllykoski, Matti
dc.contributor.author	Kursula, Inari
dc.contributor.author	Kursula, Petri
dc.date.accessioned	2017-06-21T14:46:09Z
dc.date.available	2017-06-21T14:46:09Z
dc.date.issued	2017-05-16
dc.identifier.citation	Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase. 2017, 18 (1):7 BMC Biochem.	en
dc.identifier.issn	1471-2091
dc.identifier.pmid	28511668
dc.identifier.doi	10.1186/s12858-017-0084-2
dc.identifier.uri	http://hdl.handle.net/10033/620972
dc.description.abstract	Eukaryotic tRNA splicing is an essential process in the transformation of a primary tRNA transcript into a mature functional tRNA molecule. 5'-phosphate ligation involves two steps: a healing reaction catalyzed by polynucleotide kinase (PNK) in association with cyclic phosphodiesterase (CPDase), and a sealing reaction catalyzed by an RNA ligase. The enzymes that catalyze tRNA healing in yeast and higher eukaryotes are homologous to the members of the 2H phosphoesterase superfamily, in particular to the vertebrate myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase).
dc.language.iso	en	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.title	Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	BMC biochemistry	en
refterms.dateFOA	2018-06-12T21:37:04Z
html.description.abstract	Eukaryotic tRNA splicing is an essential process in the transformation of a primary tRNA transcript into a mature functional tRNA molecule. 5'-phosphate ligation involves two steps: a healing reaction catalyzed by polynucleotide kinase (PNK) in association with cyclic phosphodiesterase (CPDase), and a sealing reaction catalyzed by an RNA ligase. The enzymes that catalyze tRNA healing in yeast and higher eukaryotes are homologous to the members of the 2H phosphoesterase superfamily, in particular to the vertebrate myelin enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase).