Variability in bacterial flagella re-growth patterns after breakage.
dc.contributor.author | Paradis, Guillaume | |
dc.contributor.author | Chevance, Fabienne F V | |
dc.contributor.author | Liou, Willisa | |
dc.contributor.author | Renault, Thibaud T | |
dc.contributor.author | Hughes, Kelly T | |
dc.contributor.author | Rainville, Simon | |
dc.contributor.author | Erhardt, Marc | |
dc.date.accessioned | 2017-06-22T14:01:26Z | |
dc.date.available | 2017-06-22T14:01:26Z | |
dc.date.issued | 2017-04-28 | |
dc.identifier.citation | Variability in bacterial flagella re-growth patterns after breakage. 2017, 7 (1):1282 Sci Rep | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.pmid | 28455518 | |
dc.identifier.doi | 10.1038/s41598-017-01302-5 | |
dc.identifier.uri | http://hdl.handle.net/10033/620976 | |
dc.description.abstract | Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Variability in bacterial flagella re-growth patterns after breakage. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany. | en |
dc.identifier.journal | Scientific reports | en |
refterms.dateFOA | 2018-06-12T21:36:32Z | |
html.description.abstract | Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken. |