New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18.
dc.contributor.author | Paulus, Constanze | |
dc.contributor.author | Rebets, Yuriy | |
dc.contributor.author | Tokovenko, Bogdan | |
dc.contributor.author | Nadmid, Suvd | |
dc.contributor.author | Terekhova, Larisa P | |
dc.contributor.author | Myronovskyi, Maksym | |
dc.contributor.author | Zotchev, Sergey B | |
dc.contributor.author | Rückert, Christian | |
dc.contributor.author | Braig, Simone | |
dc.contributor.author | Zahler, Stefan | |
dc.contributor.author | Kalinowski, Jörn | |
dc.contributor.author | Luzhetskyy, Andriy N | |
dc.date.accessioned | 2017-07-05T14:47:26Z | |
dc.date.available | 2017-07-05T14:47:26Z | |
dc.date.issued | 2017-02-10 | |
dc.identifier.citation | New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18. 2017, 7:42382 Sci Rep | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.pmid | 28186197 | |
dc.identifier.doi | 10.1038/srep42382 | |
dc.identifier.uri | http://hdl.handle.net/10033/620999 | |
dc.description.abstract | Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany. | en |
dc.identifier.journal | Scientific reports | en |
refterms.dateFOA | 2018-06-12T23:31:45Z | |
html.description.abstract | Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites. |