Cryo-electron Microscopy Study of the Genome Release of the Dicistrovirus Israeli Acute Bee Paralysis Virus.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2017-02-15
Metadata
Show full item recordAbstract
Viruses of the family Dicistroviridae can cause substantial economic damage by infecting agriculturally important insects. Israeli acute bee paralysis virus (IAPV) causes honeybee colony collapse disorder in the United States. High-resolution molecular details of the genome delivery mechanism of dicistroviruses are unknown. Here we present a cryo-electron microscopy analysis of IAPV virions induced to release their genomes in vitro We determined structures of full IAPV virions primed to release their genomes to a resolution of 3.3 Å and of empty capsids to a resolution of 3.9 Å. We show that IAPV does not form expanded A particles before genome release as in the case of related enteroviruses of the family Picornaviridae The structural changes observed in the empty IAPV particles include detachment of the VP4 minor capsid proteins from the inner face of the capsid and partial loss of the structure of the N-terminal arms of the VP2 capsid proteins. Unlike the case for many picornaviruses, the empty particles of IAPV are not expanded relative to the native virions and do not contain pores in their capsids that might serve as channels for genome release. Therefore, rearrangement of a unique region of the capsid is probably required for IAPV genome release.Citation
Cryo-electron Microscopy Study of the Genome Release of the Dicistrovirus Israeli Acute Bee Paralysis Virus. 2017, 91 (4) J. Virol.Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.Journal
Journal of virologyPubMed ID
27928006Type
ArticleLanguage
enISSN
1098-5514ae974a485f413a2113503eed53cd6c53
10.1128/JVI.02060-16
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
Related articles
- Virion Structure of Israeli Acute Bee Paralysis Virus.
- Authors: Mullapudi E, Přidal A, Pálková L, de Miranda JR, Plevka P
- Issue date: 2016 Sep 15
- Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism.
- Authors: Kalynych S, Füzik T, Přidal A, de Miranda J, Plevka P
- Issue date: 2017 Jan 17
- Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen.
- Authors: Spurny R, Přidal A, Pálková L, Kiem HKT, de Miranda JR, Plevka P
- Issue date: 2017 Mar 15
- Assembly of recombinant Israeli Acute Paralysis Virus capsids.
- Authors: Ren J, Cone A, Willmot R, Jones IM
- Issue date: 2014
- Cryo-electron microscopy reconstructions of triatoma virus particles: a clue to unravel genome delivery and capsid disassembly.
- Authors: Agirre J, Goret G, LeGoff M, Sánchez-Eugenia R, Marti GA, Navaza J, Guérin DMA, Neumann E
- Issue date: 2013 May