Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms.
Microsoft Excel 2007
supplementary table S6
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThis study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.
CitationCharacterisation of a stable laboratory co-culture of acidophilic nanoorganisms. 2017, 7 (1):3289 Sci Rep
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Cuniculiplasmataceae, their ecogenomic and metabolic patterns, and interactions with 'ARMAN'.
- Authors: Golyshina OV, Bargiela R, Golyshin PN
- Issue date: 2019 Jan
- Metabolic Diversity and Evolutionary History of the Archaeal Phylum "<i>Candidatus</i> Micrarchaeota" Uncovered from a Freshwater Lake Metagenome.
- Authors: Kadnikov VV, Savvichev AS, Mardanov AV, Beletsky AV, Chupakov AV, Kokryatskaya NM, Pimenov NV, Ravin NV
- Issue date: 2020 Nov 10
- 'ARMAN' archaea depend on association with euryarchaeal host in culture and in situ.
- Authors: Golyshina OV, Toshchakov SV, Makarova KS, Gavrilov SN, Korzhenkov AA, La Cono V, Arcadi E, Nechitaylo TY, Ferrer M, Kublanov IV, Wolf YI, Yakimov MM, Golyshin PN
- Issue date: 2017 Jul 5
- Diversity of "<i>Ca</i>. Micrarchaeota" in Two Distinct Types of Acidic Environments and Their Associations with <i>Thermoplasmatales</i>.
- Authors: Golyshina OV, Bargiela R, Toshchakov SV, Chernyh NA, Ramayah S, Korzhenkov AA, Kublanov IV, Golyshin PN
- Issue date: 2019 Jun 15
- Oxygen-dependent niche formation of a pyrite-dependent acidophilic consortium built by archaea and bacteria.
- Authors: Ziegler S, Dolch K, Geiger K, Krause S, Asskamp M, Eusterhues K, Kriews M, Wilhelms-Dick D, Goettlicher J, Majzlan J, Gescher J
- Issue date: 2013 Sep