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dc.contributor.authorChao, Yanjie
dc.contributor.authorLi, Lei
dc.contributor.authorGirodat, Dylan
dc.contributor.authorFörstner, Konrad U
dc.contributor.authorSaid, Nelly
dc.contributor.authorCorcoran, Colin
dc.contributor.authorŚmiga, Michał
dc.contributor.authorPapenfort, Kai
dc.contributor.authorReinhardt, Richard
dc.contributor.authorWieden, Hans-Joachim
dc.contributor.authorLuisi, Ben F
dc.contributor.authorVogel, Jörg
dc.date.accessioned2017-09-06T09:27:44Z
dc.date.available2017-09-06T09:27:44Z
dc.date.issued2017-01-05
dc.identifier.citationIn Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways. 2017, 65 (1):39-51 Mol. Cellen
dc.identifier.issn1097-4164
dc.identifier.pmid28061332
dc.identifier.doi10.1016/j.molcel.2016.11.002
dc.identifier.urihttp://hdl.handle.net/10033/621094
dc.description.abstractUnderstanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.en
dc.typeArticleen
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße 2, 97080 Würzburg, Germany.en
dc.identifier.journalMolecular cellen
refterms.dateFOA2018-01-05T00:00:00Z
html.description.abstractUnderstanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.


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