In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.
dc.contributor.author | Chao, Yanjie | |
dc.contributor.author | Li, Lei | |
dc.contributor.author | Girodat, Dylan | |
dc.contributor.author | Förstner, Konrad U | |
dc.contributor.author | Said, Nelly | |
dc.contributor.author | Corcoran, Colin | |
dc.contributor.author | Śmiga, Michał | |
dc.contributor.author | Papenfort, Kai | |
dc.contributor.author | Reinhardt, Richard | |
dc.contributor.author | Wieden, Hans-Joachim | |
dc.contributor.author | Luisi, Ben F | |
dc.contributor.author | Vogel, Jörg | |
dc.date.accessioned | 2017-09-06T09:27:44Z | |
dc.date.available | 2017-09-06T09:27:44Z | |
dc.date.issued | 2017-01-05 | |
dc.identifier.citation | In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways. 2017, 65 (1):39-51 Mol. Cell | en |
dc.identifier.issn | 1097-4164 | |
dc.identifier.pmid | 28061332 | |
dc.identifier.doi | 10.1016/j.molcel.2016.11.002 | |
dc.identifier.uri | http://hdl.handle.net/10033/621094 | |
dc.description.abstract | Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways. | en |
dc.type | Article | en |
dc.contributor.department | HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße 2, 97080 Würzburg, Germany. | en |
dc.identifier.journal | Molecular cell | en |
refterms.dateFOA | 2018-01-05T00:00:00Z | |
html.description.abstract | Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators. |