Tissue dual RNA-seq allows fast discovery of infection-specific functions and riboregulators shaping host-pathogen transcriptomes.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsNuss, Aaron M
Heroven, Ann Kathrin
MetadataShow full item record
AbstractPathogenic bacteria need to rapidly adjust their virulence and fitness program to prevent eradication by the host. So far, underlying adaptation processes that drive pathogenesis have mostly been studied in vitro, neglecting the true complexity of host-induced stimuli acting on the invading pathogen. In this study, we developed an unbiased experimental approach that allows simultaneous monitoring of genome-wide infection-linked transcriptional alterations of the host and colonizing extracellular pathogens. Using this tool for Yersinia pseudotuberculosis-infected lymphatic tissues, we revealed numerous alterations of host transcripts associated with inflammatory and acute-phase responses, coagulative activities, and transition metal ion sequestration, highlighting that the immune response is dominated by infiltrating neutrophils and elicits a mixed TH17/TH1 response. In consequence, the pathogen's response is mainly directed to prevent phagocytic attacks. Yersinia up-regulates the gene and expression dose of the antiphagocytic type III secretion system (T3SS) and induces functions counteracting neutrophil-induced ion deprivation, radical stress, and nutritional restraints. Several conserved bacterial riboregulators were identified that impacted this response. The strongest influence on virulence was found for the loss of the carbon storage regulator (Csr) system, which is shown to be essential for the up-regulation of the T3SS on host cell contact. In summary, our established approach provides a powerful tool for the discovery of infection-specific stimuli, induced host and pathogen responses, and underlying regulatory processes.
CitationTissue dual RNA-seq allows fast discovery of infection-specific functions and riboregulators shaping host-pathogen transcriptomes. 2017, 114 (5):E791-E800 Proc. Natl. Acad. Sci. U.S.A.
AffiliationHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Discovering Yersinia-Host Interactions by Tissue Dual RNA-Seq.
- Authors: Kusmierek M, Heroven AK, Beckstette M, Nuss AM, Dersch P
- Issue date: 2019
- IscR is essential for yersinia pseudotuberculosis type III secretion and virulence.
- Authors: Miller HK, Kwuan L, Schwiesow L, Bernick DL, Mettert E, Ramirez HA, Ragle JM, Chan PP, Kiley PJ, Lowe TM, Auerbuch V
- Issue date: 2014 Jun
- Impact of CCR7 on T-Cell Response and Susceptibility to Yersinia pseudotuberculosis Infection.
- Authors: Pezoldt J, Pisano F, Heine W, Pasztoi M, Rosenheinrich M, Nuss AM, Pils MC, Prinz I, Förster R, Huehn J, Dersch P
- Issue date: 2017 Sep 15
- Genome Scale Analysis Reveals IscR Directly and Indirectly Regulates Virulence Factor Genes in Pathogenic <i>Yersinia</i>.
- Authors: Balderas D, Mettert E, Lam HN, Banerjee R, Gverzdys T, Alvarez P, Saarunya G, Tanner N, Zoubedi A, Wei Y, Kiley PJ, Auerbuch V
- Issue date: 2021 Jun 29
- Toll-like receptor 2 is critical for induction of Reg3 beta expression and intestinal clearance of Yersinia pseudotuberculosis.
- Authors: Dessein R, Gironella M, Vignal C, Peyrin-Biroulet L, Sokol H, Secher T, Lacas-Gervais S, Gratadoux JJ, Lafont F, Dagorn JC, Ryffel B, Akira S, Langella P, Nùñez G, Sirard JC, Iovanna J, Simonet M, Chamaillard M
- Issue date: 2009 Jun