Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074.
Name:
Ahmed and Luzhetskyy.pdf
Size:
2.658Mb
Format:
PDF
Description:
Open Access publication
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2017-08-29
Metadata
Show full item recordAbstract
A large majority of genome-encrypted chemical diversity in actinobacteria remains to be discovered, which is related to the low level of secondary metabolism genes expression. Here, we report the application of a reporter-guided screening strategy to activate cryptic polycyclic tetramate macrolactam gene clusters in Streptomyces albus J1074. The analysis of the S. albus transcriptome revealed an overall low level of secondary metabolism genes transcription. Combined with transposon mutagenesis, reporter-guided screening resulted in the selection of two S. albus strains with altered secondary metabolites production. Transposon insertion in the most prominent strain, S. albus ATGSal2P2::TN14, was mapped to the XNR_3174 gene encoding an unclassified transcriptional regulator. The mutant strain was found to produce the avenolide-like compound butenolide 4. The deletion of the gene encoding a putative acyl-CoA oxidase, an orthologue of the Streptomyces avermitilis avenolide biosynthesis enzyme, in the S. albus XNR_3174 mutant caused silencing of secondary metabolism. The homologues of XNR_3174 and the butenolide biosynthesis genes were found in the genomes of multiple Streptomyces species. This result leads us to believe that the discovered regulatory elements comprise a new condition-dependent system that controls secondary metabolism in actinobacteria and can be manipulated to activate cryptic biosynthetic pathways.Citation
Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074. 2017, 7 (1):9784 Sci RepAffiliation
Helmholtz-Institut für pharmazeutische Forschung Saarland,Universitätscampus E8.1, 66123 Saarbrücken, Germany.Journal
Scientific reportsPubMed ID
28852167Type
ArticleLanguage
enISSN
2045-2322ae974a485f413a2113503eed53cd6c53
10.1038/s41598-017-10316-y
Scopus Count
The following license files are associated with this item:
- Creative Commons
Related articles
- Butenolides from Streptomyces albus J1074 Act as External Signals To Stimulate Avermectin Production in Streptomyces avermitilis.
- Authors: Nguyen TB, Kitani S, Shimma S, Nihira T
- Issue date: 2018 May 1
- Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis.
- Authors: Sultan SP, Kitani S, Miyamoto KT, Iguchi H, Atago T, Ikeda H, Nihira T
- Issue date: 2016 Nov
- Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074.
- Authors: Olano C, García I, González A, Rodriguez M, Rozas D, Rubio J, Sánchez-Hidalgo M, Braña AF, Méndez C, Salas JA
- Issue date: 2014 May
- Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.
- Authors: Bilyk B, Horbal L, Luzhetskyy A
- Issue date: 2017 Jan 4
- Activation and silencing of secondary metabolites in Streptomyces albus and Streptomyces lividans after transformation with cosmids containing the thienamycin gene cluster from Streptomyces cattleya.
- Authors: Braña AF, Rodríguez M, Pahari P, Rohr J, García LA, Blanco G
- Issue date: 2014 May