Show simple item record

dc.contributor.authorNonnenmacher, Yannic
dc.contributor.authorPalorini, Roberta
dc.contributor.authord'Herouël, Aymeric Fouquier
dc.contributor.authorKrämer, Lisa
dc.contributor.authorNeumann-Schaal, Meina
dc.contributor.authorChiaradonna, Ferdinando
dc.contributor.authorSkupin, Alexander
dc.contributor.authorWegner, Andre
dc.contributor.authorHiller, Karsten
dc.date.accessioned2017-10-06T09:18:42Z
dc.date.available2017-10-06T09:18:42Z
dc.date.issued2017-09
dc.identifier.citationAnalysis of mitochondrial metabolism in situ: Combining stable isotope labeling with selective permeabilization. 2017, 43 (Pt B):147-155 Metab. Eng.en
dc.identifier.issn1096-7184
dc.identifier.pmid27988388
dc.identifier.doi10.1016/j.ymben.2016.12.005
dc.identifier.urihttp://hdl.handle.net/10033/621128
dc.description.abstractTo date, it is well-established that mitochondrial dysfunction does not only play a vital role in cancer but also in other pathological conditions such as neurodegenerative diseases and inflammation. An important tool for the analysis of cellular metabolism is the application of stable isotope labeled substrates, which allow for the tracing of atoms throughout metabolic networks. While such analyses yield very detailed information about intracellular fluxes, the determination of compartment specific fluxes is far more challenging. Most approaches for the deconvolution of compartmented metabolism use computational models whereas experimental methods are rare. Here, we developed an experimental setup based on selective permeabilization of the cytosolic membrane that allows for the administration of stable isotope labeled substrates directly to mitochondria. We demonstrate how this approach can be used to infer metabolic changes in mitochondria induced by either chemical or genetic perturbations and give an outlook on its potential applications.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAnalysis of mitochondrial metabolism in situ: Combining stable isotope labeling with selective permeabilization.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalMetabolic engineeringen
refterms.dateFOA2018-06-12T22:56:20Z
html.description.abstractTo date, it is well-established that mitochondrial dysfunction does not only play a vital role in cancer but also in other pathological conditions such as neurodegenerative diseases and inflammation. An important tool for the analysis of cellular metabolism is the application of stable isotope labeled substrates, which allow for the tracing of atoms throughout metabolic networks. While such analyses yield very detailed information about intracellular fluxes, the determination of compartment specific fluxes is far more challenging. Most approaches for the deconvolution of compartmented metabolism use computational models whereas experimental methods are rare. Here, we developed an experimental setup based on selective permeabilization of the cytosolic membrane that allows for the administration of stable isotope labeled substrates directly to mitochondria. We demonstrate how this approach can be used to infer metabolic changes in mitochondria induced by either chemical or genetic perturbations and give an outlook on its potential applications.


Files in this item

Thumbnail
Name:
Publisher version
Thumbnail
Name:
Nonnenmacher et al.pdf
Size:
823.9Kb
Format:
PDF
Description:
Open Access article

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by-nc-sa/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/