Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp. massiliense CRM0019 in alveolar epithelial cells.

Ribeiro, Giovanni Monteiro; Matsumoto, Cristianne Kayoko; Real, Fernando; Teixeira, Daniela; Duarte, Rafael Silva; Mortara, Renato Arruda; Leão, Sylvia Cardoso; de Souza Carvalho-Wodarz, Cristiane

dc.contributor.author	Ribeiro, Giovanni Monteiro
dc.contributor.author	Matsumoto, Cristianne Kayoko
dc.contributor.author	Real, Fernando
dc.contributor.author	Teixeira, Daniela
dc.contributor.author	Duarte, Rafael Silva
dc.contributor.author	Mortara, Renato Arruda
dc.contributor.author	Leão, Sylvia Cardoso
dc.contributor.author	de Souza Carvalho-Wodarz, Cristiane
dc.date.accessioned	2017-10-24T08:43:03Z
dc.date.available	2017-10-24T08:43:03Z
dc.date.issued	2017-09-13
dc.identifier.citation	Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp. massiliense CRM0019 in alveolar epithelial cells. 2017, 17 (1):195 BMC Microbiol.	en
dc.identifier.issn	1471-2180
dc.identifier.pmid	28903728
dc.identifier.doi	10.1186/s12866-017-1102-7
dc.identifier.uri	http://hdl.handle.net/10033/621143
dc.description.abstract	Outbreaks of infections caused by rapidly growing mycobacteria have been reported worldwide generally associated with medical procedures. Mycobacterium abscessus subsp. massiliense CRM0019 was obtained during an epidemic of postsurgical infections and was characterized by increased persistence in vivo. To better understand the successful survival strategies of this microorganism, we evaluated its infectivity and proliferation in macrophages (RAW and BMDM) and alveolar epithelial cells (A549). For that, we assessed the following parameters, for both M. abscessus CRM0019 as well as the reference strain M. abscessus ATCC 19977: internalization, intracellular survival for up 3 days, competence to subvert lysosome fusion and the intracellular survival after cell reinfection.
dc.language.iso	en	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.title	Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp. massiliense CRM0019 in alveolar epithelial cells.	en
dc.type	Article	en
dc.contributor.department	Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Universitycampus E8.1, 66123 Saarbrücken, Germany.	en
dc.identifier.journal	BMC microbiology	en
refterms.dateFOA	2018-06-12T23:39:29Z
html.description.abstract	Outbreaks of infections caused by rapidly growing mycobacteria have been reported worldwide generally associated with medical procedures. Mycobacterium abscessus subsp. massiliense CRM0019 was obtained during an epidemic of postsurgical infections and was characterized by increased persistence in vivo. To better understand the successful survival strategies of this microorganism, we evaluated its infectivity and proliferation in macrophages (RAW and BMDM) and alveolar epithelial cells (A549). For that, we assessed the following parameters, for both M. abscessus CRM0019 as well as the reference strain M. abscessus ATCC 19977: internalization, intracellular survival for up 3 days, competence to subvert lysosome fusion and the intracellular survival after cell reinfection.