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dc.contributor.authorBoedeker, Christian
dc.contributor.authorSchüler, Margarete
dc.contributor.authorReintjes, Greta
dc.contributor.authorJeske, Olga
dc.contributor.authorvan Teeseling, Muriel C F
dc.contributor.authorJogler, Mareike
dc.contributor.authorRast, Patrick
dc.contributor.authorBorchert, Daniela
dc.contributor.authorDevos, Damien P
dc.contributor.authorKucklick, Martin
dc.contributor.authorSchaffer, Miroslava
dc.contributor.authorKolter, Roberto
dc.contributor.authorvan Niftrik, Laura
dc.contributor.authorEngelmann, Susanne
dc.contributor.authorAmann, Rudolf
dc.contributor.authorRohde, M
dc.contributor.authorEngelhardt, Harald
dc.contributor.authorJogler, Christian
dc.date.accessioned2017-12-20T14:47:25Z
dc.date.available2017-12-20T14:47:25Z
dc.date.issued2017-04-10
dc.identifier.citationDetermining the bacterial cell biology of Planctomycetes. 2017, 8:14853 Nat Communen
dc.identifier.issn2041-1723
dc.identifier.pmid28393831
dc.identifier.doi10.1038/ncomms14853
dc.identifier.urihttp://hdl.handle.net/10033/621214
dc.description.abstractBacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDetermining the bacterial cell biology of Planctomycetes.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalNature communicationsen
refterms.dateFOA2018-06-12T20:00:26Z
html.description.abstractBacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.


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