Supplementary figures Babdor et ...
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Adiko, Aimé Cézaire
Vasconcellos, Luiz Ricardo
De Luca, Mariacristina
Brinkmann, Melanie M
MetadataShow full item record
AbstractThe retention of intracellular Toll-like receptors (TLRs) in the endoplasmic reticulum prevents their activation under basal conditions. TLR9 is activated by sensing ligands in specific endosomal-lysosomal compartments. Here we identified IRAP+ endosomes as major cellular compartments for the early steps of TLR9 activation in dendritic cells (DCs). Both TLR9 and its ligand, the dinucleotide CpG, were present as cargo in IRAP+ endosomes. In the absence of the aminopeptidase IRAP, the trafficking of CpG and TLR9 to lysosomes and signaling via TLR9 were enhanced in DCs and in mice following bacterial infection. IRAP stabilized CpG-containing endosomes by interacting with the actin-nucleation factor FHOD4, which slowed the trafficking of TLR9 toward lysosomes. Thus, endosomal retention of TLR9 via the interaction of IRAP with the actin cytoskeleton is a mechanism that prevents hyper-activation of TLR9 in DCs.
CitationIRAP+ endosomes restrict TLR9 activation and signaling. 2017, 18 (5):509-518 Nat. Immunol.
AffiliationHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
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- Issue date: 2015 Jun 1
- Endosomal translocation of vertebrate DNA activates dendritic cells via TLR9-dependent and -independent pathways.
- Authors: Yasuda K, Yu P, Kirschning CJ, Schlatter B, Schmitz F, Heit A, Bauer S, Hochrein H, Wagner H
- Issue date: 2005 May 15
- CpG-induced tyrosine phosphorylation occurs via a TLR9-independent mechanism and is required for cytokine secretion.
- Authors: Sanjuan MA, Rao N, Lai KT, Gu Y, Sun S, Fuchs A, Fung-Leung WP, Colonna M, Karlsson L
- Issue date: 2006 Mar 27
- Toll-like receptor 9 trafficking and signaling for type I interferons requires PIKfyve activity.
- Authors: Hayashi K, Sasai M, Iwasaki A
- Issue date: 2015 Sep
- CpG motif-independent activation of TLR9 upon endosomal translocation of "natural" phosphodiester DNA.
- Authors: Yasuda K, Rutz M, Schlatter B, Metzger J, Luppa PB, Schmitz F, Haas T, Heit A, Bauer S, Wagner H
- Issue date: 2006 Feb