Show simple item record

dc.contributor.authorGálvez, Eric J C
dc.contributor.authorIljazovic, Aida
dc.contributor.authorGronow, Achim
dc.contributor.authorFlavell, Richard
dc.contributor.authorStrowig, Till
dc.date.accessioned2018-01-18T14:56:35Z
dc.date.available2018-01-18T14:56:35Z
dc.date.issued2017-12-26
dc.identifier.citationShaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure. 2017, 21 (13):3914-3926 Cell Repen
dc.identifier.issn2211-1247
dc.identifier.pmid29281837
dc.identifier.doi10.1016/j.celrep.2017.12.027
dc.identifier.urihttp://hdl.handle.net/10033/621241
dc.description.abstractContradicting observations have been made regarding the relative contributions of immune sensors to shaping the microbiome, yet the reasons for these discrepancies are not fully understood. Here, we investigated the contribution of environmental factors in shaping the microbiome in mice deficient in adaptive immunity (Rag2-/-) and Nlrp6, an immune sensor proposed to be involved in regulation of microbiota composition. In conventionally housed Nlrp6-/- mice, familial transmission has a significant effect on microbiota composition, complicating the analysis of genotype-dependent effects. Notably, after rederivation into standardized specific pathogen-free (SPF) conditions devoid of pathobionts, microbiota composition was indistinguishable between WT, Rag2-/-, and Nlrp6-/- mice. However, upon reintroduction of a pathobiont-containing community host, genotype-dependent differences reappear, specifically affecting the relative abundance of pathobionts such as Helicobacter spp. Our results show that the impact of Nlrp6 and also of adaptive immunity on microbiota composition depends on community structure and primarily influences pathobionts but not commensals.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleShaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalCell reportsen
refterms.dateFOA2018-06-12T22:08:41Z
html.description.abstractContradicting observations have been made regarding the relative contributions of immune sensors to shaping the microbiome, yet the reasons for these discrepancies are not fully understood. Here, we investigated the contribution of environmental factors in shaping the microbiome in mice deficient in adaptive immunity (Rag2-/-) and Nlrp6, an immune sensor proposed to be involved in regulation of microbiota composition. In conventionally housed Nlrp6-/- mice, familial transmission has a significant effect on microbiota composition, complicating the analysis of genotype-dependent effects. Notably, after rederivation into standardized specific pathogen-free (SPF) conditions devoid of pathobionts, microbiota composition was indistinguishable between WT, Rag2-/-, and Nlrp6-/- mice. However, upon reintroduction of a pathobiont-containing community host, genotype-dependent differences reappear, specifically affecting the relative abundance of pathobionts such as Helicobacter spp. Our results show that the impact of Nlrp6 and also of adaptive immunity on microbiota composition depends on community structure and primarily influences pathobionts but not commensals.


Files in this item

Thumbnail
Name:
Publisher version
Thumbnail
Name:
Gálvez et al.pdf
Size:
4.835Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

http://creativecommons.org/licenses/by-nc-sa/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/