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Increased plasmid copy number is essential for Yersinia T3SS function and virulence.

dc.contributor.authorWang, He
dc.contributor.authorAvican, Kemal
dc.contributor.authorFahlgren, Anna
dc.contributor.authorErttmann, Saskia F
dc.contributor.authorNuss, Aaron M
dc.contributor.authorDersch, Petra
dc.contributor.authorFallman, Maria
dc.contributor.authorEdgren, Tomas
dc.contributor.authorWolf-Watz, Hans
dc.date.accessioned2018-02-06T09:35:50Z
dc.date.available2018-02-06T09:35:50Z
dc.date.issued2016-07-29
dc.identifier.citationIncreased plasmid copy number is essential for Yersinia T3SS function and virulence. 2016, 353 (6298):492-5 Scienceen
dc.identifier.issn1095-9203
dc.identifier.pmid27365311
dc.identifier.doi10.1126/science.aaf7501
dc.identifier.urihttp://hdl.handle.net/10033/621262
dc.description.abstractPathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS. Remarkably, we observed direct, tight coupling between plasmid replication and T3SS function. This regulatory pathway provides a framework for further exploration of the environmental sensing mechanisms of pathogenic bacteria.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalsen
dc.subject.meshGene Dosageen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshPlasmidsen
dc.subject.meshType III Secretion Systemsen
dc.subject.meshVirulenceen
dc.subject.meshVirulence Factorsen
dc.subject.meshYersinia pseudotuberculosisen
dc.subject.meshYersinia pseudotuberculosis Infectionsen
dc.titleIncreased plasmid copy number is essential for Yersinia T3SS function and virulence.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsfoschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalScience (New York, N.Y.)en
refterms.dateFOA2018-06-13T21:22:08Z
html.description.abstractPathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS. Remarkably, we observed direct, tight coupling between plasmid replication and T3SS function. This regulatory pathway provides a framework for further exploration of the environmental sensing mechanisms of pathogenic bacteria.


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