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dc.contributor.authorLeithner, Alexander
dc.contributor.authorEichner, Alexander
dc.contributor.authorMüller, Jan
dc.contributor.authorReversat, Anne
dc.contributor.authorBrown, Markus
dc.contributor.authorSchwarz, Jan
dc.contributor.authorMerrin, Jack
dc.contributor.authorde Gorter, David J J
dc.contributor.authorSchur, Florian
dc.contributor.authorBayerl, Jonathan
dc.contributor.authorde Vries, Ingrid
dc.contributor.authorWieser, Stefan
dc.contributor.authorHauschild, Robert
dc.contributor.authorLai, Frank P L
dc.contributor.authorMoser, Markus
dc.contributor.authorKerjaschki, Dontscho
dc.contributor.authorRottner, Klemens
dc.contributor.authorSmall, J Victor
dc.contributor.authorStradal, Theresia E B
dc.contributor.authorSixt, Michael
dc.date.accessioned2018-02-09T15:48:16Z
dc.date.available2018-02-09T15:48:16Z
dc.date.issued2016-11
dc.identifier.citationDiversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. 2016, 18 (11):1253-1259 Nat. Cell Biol.en
dc.identifier.issn1476-4679
dc.identifier.pmid27775702
dc.identifier.doi10.1038/ncb3426
dc.identifier.urihttp://hdl.handle.net/10033/621275
dc.description.abstractMost migrating cells extrude their front by the force of actin polymerization. Polymerization requires an initial nucleation step, which is mediated by factors establishing either parallel filaments in the case of filopodia or branched filaments that form the branched lamellipodial network. Branches are considered essential for regular cell motility and are initiated by the Arp2/3 complex, which in turn is activated by nucleation-promoting factors of the WASP and WAVE families. Here we employed rapid amoeboid crawling leukocytes and found that deletion of the WAVE complex eliminated actin branching and thus lamellipodia formation. The cells were left with parallel filaments at the leading edge, which translated, depending on the differentiation status of the cell, into a unipolar pointed cell shape or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased speed and enormous directional persistence, while they were unable to turn towards chemotactic gradients. Cells with multiple filopodia retained chemotactic activity but their migration was progressively impaired with increasing geometrical complexity of the extracellular environment. These findings establish that diversified leading edge protrusions serve as explorative structures while they slow down actual locomotion.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshActin Cytoskeletonen
dc.subject.meshActin-Related Protein 2-3 Complexen
dc.subject.meshActinsen
dc.subject.meshAnimalsen
dc.subject.meshCell Movementen
dc.subject.meshDendritic Cellsen
dc.subject.meshLeukocytesen
dc.subject.meshMiceen
dc.subject.meshMice, Knockouten
dc.subject.meshPolymerizationen
dc.subject.meshPseudopodiaen
dc.titleDiversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes.en
dc.typeArticleen
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynnen-Str.7, 30625 Hannover, Germany.en
dc.identifier.journalNature cell biologyen
refterms.dateFOA2018-06-13T09:26:41Z
html.description.abstractMost migrating cells extrude their front by the force of actin polymerization. Polymerization requires an initial nucleation step, which is mediated by factors establishing either parallel filaments in the case of filopodia or branched filaments that form the branched lamellipodial network. Branches are considered essential for regular cell motility and are initiated by the Arp2/3 complex, which in turn is activated by nucleation-promoting factors of the WASP and WAVE families. Here we employed rapid amoeboid crawling leukocytes and found that deletion of the WAVE complex eliminated actin branching and thus lamellipodia formation. The cells were left with parallel filaments at the leading edge, which translated, depending on the differentiation status of the cell, into a unipolar pointed cell shape or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased speed and enormous directional persistence, while they were unable to turn towards chemotactic gradients. Cells with multiple filopodia retained chemotactic activity but their migration was progressively impaired with increasing geometrical complexity of the extracellular environment. These findings establish that diversified leading edge protrusions serve as explorative structures while they slow down actual locomotion.


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