Archaea box C/D enzymes methylate two distinct substrate rRNA sequences with different efficiency.
-Graziadei et al.pdf
after embargo allowed publisher's ...
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractRNA modifications confer complexity to the 4-nucleotide polymer; nevertheless, their exact function is mostly unknown. rRNA 2'-O-ribose methylation concentrates to ribosome functional sites and is important for ribosome biogenesis. The methyl group is transferred to rRNA by the box C/D RNPs: The rRNA sequence to be methylated is recognized by a complementary sequence on the guide RNA, which is part of the enzyme. In contrast to their eukaryotic homologs, archaeal box C/D enzymes can be assembled in vitro and are used to study the mechanism of 2'-O-ribose methylation. In Archaea, each guide RNA directs methylation to two distinct rRNA sequences, posing the question whether this dual architecture of the enzyme has a regulatory role. Here we use methylation assays and low-resolution structural analysis with small-angle X-ray scattering to study the methylation reaction guided by the sR26 guide RNA fromPyrococcus furiosus We find that the methylation efficacy at sites D and D' differ substantially, with substrate D' turning over more efficiently than substrate D. This observation correlates well with structural data: The scattering profile of the box C/D RNP half-loaded with substrate D' is similar to that of the holo complex, which has the highest activity. Unexpectedly, the guide RNA secondary structure is not responsible for the functional difference at the D and D' sites. Instead, this difference is recapitulated by the nature of the first base pair of the guide-substrate duplex. We suggest that substrate turnover may occur through a zip mechanism that initiates at the 5'-end of the product.
CitationArchaea box C/D enzymes methylate two distinct substrate rRNA sequences with different efficiency. 2016, 22 (5):764-72 RNA
AffiliationHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
JournalRNA (New York, N.Y.)
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- C/D box sRNA-guided 2'-O-methylation patterns of archaeal rRNA molecules.
- Authors: Dennis PP, Tripp V, Lui L, Lowe T, Randau L
- Issue date: 2015 Aug 22
- Archaeal homologs of eukaryotic methylation guide small nucleolar RNAs: lessons from the Pyrococcus genomes.
- Authors: Gaspin C, Cavaillé J, Erauso G, Bachellerie JP
- Issue date: 2000 Apr 7
- Efficient RNA 2'-O-methylation requires juxtaposed and symmetrically assembled archaeal box C/D and C'/D' RNPs.
- Authors: Tran EJ, Zhang X, Maxwell ES
- Issue date: 2003 Aug 1
- RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea.
- Authors: Ziesche SM, Omer AD, Dennis PP
- Issue date: 2004 Nov
- Structural features of the guide:target RNA duplex required for archaeal box C/D sRNA-guided nucleotide 2'-O-methylation.
- Authors: Appel CD, Maxwell ES
- Issue date: 2007 Jun