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dc.contributor.authorSpring, Stefan
dc.contributor.authorBunk, Boyke
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorSchumann, Peter
dc.contributor.authorRohde, M
dc.contributor.authorTindall, Brian J
dc.contributor.authorKlenk, Hans-Peter
dc.date.accessioned2018-02-26T10:15:23Z
dc.date.available2018-02-26T10:15:23Z
dc.date.issued2016
dc.identifier.citationCharacterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum. 2016, 10 (12):2801-2816 ISME Jen
dc.identifier.issn1751-7370
dc.identifier.pmid27300277
dc.identifier.doi10.1038/ismej.2016.84
dc.identifier.urihttp://hdl.handle.net/10033/621297
dc.description.abstractThe recently isolated strain L21-Fru-ABTrepresents moderately halophilic, obligately anaerobic and saccharolytic bacteria that thrive in the suboxic transition zones of hypersaline microbial mats. Phylogenetic analyses based on 16S rRNA genes, RpoB proteins and gene content indicated that strain L21-Fru-ABTrepresents a novel species and genus affiliated with a distinct phylum-level lineage originally designated Verrucomicrobia subdivision 5. A survey of environmental 16S rRNA gene sequences revealed that members of this newly recognized phylum are wide-spread and ecologically important in various anoxic environments ranging from hypersaline sediments to wastewater and the intestine of animals. Characteristic phenotypic traits of the novel strain included the formation of extracellular polymeric substances, a Gram-negative cell wall containing peptidoglycan and the absence of odd-numbered cellular fatty acids. Unusual metabolic features deduced from analysis of the genome sequence were the production of sucrose as osmoprotectant, an atypical glycolytic pathway lacking pyruvate kinase and the synthesis of isoprenoids via mevalonate. On the basis of the analyses of phenotypic, genomic and environmental data, it is proposed that strain L21-Fru-ABTand related bacteria are specifically adapted to the utilization of sulfated glycopolymers produced in microbial mats or biofilms.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshBacterial Proteinsen
dc.subject.meshBacterial Typing Techniquesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshFatty Acidsen
dc.subject.meshLakesen
dc.subject.meshPhylogenyen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshVerrucomicrobiaen
dc.titleCharacterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalThe ISME journalen
refterms.dateFOA2018-06-13T19:31:31Z
html.description.abstractThe recently isolated strain L21-Fru-ABTrepresents moderately halophilic, obligately anaerobic and saccharolytic bacteria that thrive in the suboxic transition zones of hypersaline microbial mats. Phylogenetic analyses based on 16S rRNA genes, RpoB proteins and gene content indicated that strain L21-Fru-ABTrepresents a novel species and genus affiliated with a distinct phylum-level lineage originally designated Verrucomicrobia subdivision 5. A survey of environmental 16S rRNA gene sequences revealed that members of this newly recognized phylum are wide-spread and ecologically important in various anoxic environments ranging from hypersaline sediments to wastewater and the intestine of animals. Characteristic phenotypic traits of the novel strain included the formation of extracellular polymeric substances, a Gram-negative cell wall containing peptidoglycan and the absence of odd-numbered cellular fatty acids. Unusual metabolic features deduced from analysis of the genome sequence were the production of sucrose as osmoprotectant, an atypical glycolytic pathway lacking pyruvate kinase and the synthesis of isoprenoids via mevalonate. On the basis of the analyses of phenotypic, genomic and environmental data, it is proposed that strain L21-Fru-ABTand related bacteria are specifically adapted to the utilization of sulfated glycopolymers produced in microbial mats or biofilms.


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