Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsRomero Pastrana, Francisco
Koedijk, Dennis G A M
de Graaf, Douwe
Jonkman, Marcel F
van Dijl, Jan Maarten
MetadataShow full item record
AbstractHuman antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antigens for vaccination. The present study was aimed at assessing immunoglobulin G (IgG) responses elicited by non-covalently cell surface-bound proteins of S. aureus, which thus far received relatively little attention. To this end, we applied plasma samples from patients with the genetic blistering disease epidermolysis bullosa (EB) and healthy S. aureus carriers. Of note, wounds of EB patients are highly colonized with S. aureus and accordingly these patients are more seriously exposed to staphylococcal antigens than healthy individuals. Ten non-covalently cell surface-bound proteins of S. aureus, namely Atl, Eap, Efb, EMP, IsaA, LukG, LukH, SA0710, Sle1 and SsaA2, were selected by bioinformatics and biochemical approaches. These antigens were recombinantly expressed, purified and tested for specific IgG responses using human plasma. We show that high exposure of EB patients to S. aureus is mirrored by elevated IgG levels against all tested non-covalently cell wall-bound staphylococcal antigens. This implies that these S. aureus cell surface proteins are prime targets for the human immune system.
CitationHuman antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins. 2018, 8 (1):3234 Sci Rep
AffiliationHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- IgG4 subclass-specific responses to Staphylococcus aureus antigens shed new light on host-pathogen interaction.
- Authors: Swierstra J, Debets S, de Vogel C, Lemmens-den Toom N, Verkaik N, Ramdani-Bouguessa N, Jonkman MF, van Dijl JM, Fahal A, van Belkum A, van Wamel W
- Issue date: 2015 Feb
- Differential epitope recognition in the immunodominant staphylococcal antigen A of Staphylococcus aureus by mouse versus human IgG antibodies.
- Authors: Koedijk DGAM, Pastrana FR, Hoekstra H, Berg SVD, Back JW, Kerstholt C, Prins RC, Bakker-Woudenberg IAJM, van Dijl JM, Buist G
- Issue date: 2017 Aug 15
- Induction of antibodies by Staphylococcus aureus nasal colonization in young children.
- Authors: Verkaik NJ, Lebon A, de Vogel CP, Hooijkaas H, Verbrugh HA, Jaddoe VW, Hofman A, Moll HA, van Belkum A, van Wamel WJ
- Issue date: 2010 Aug
- Host-pathogen interactions in epidermolysis bullosa patients colonized with Staphylococcus aureus.
- Authors: van der Kooi-Pol MM, Duipmans JC, Jonkman MF, van Dijl JM
- Issue date: 2014 Mar
- Nasopharyngeal colonization elicits antibody responses to staphylococcal and pneumococcal proteins that are not associated with a reduced risk of subsequent carriage.
- Authors: Prevaes SM, van Wamel WJ, de Vogel CP, Veenhoven RH, van Gils EJ, van Belkum A, Sanders EA, Bogaert D
- Issue date: 2012 Jun