'Candidatus Adiutrix intracellularis', an endosymbiont of termite gut flagellates, is the first representative of a deep-branching clade of Deltaproteobacteria and a putative homoacetogen.
Ikeda-Ohtsubo et al.pdf
peer-reviewed accepted manuscript
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Strassert, Jürgen F H
McHardy, Alice C
Tringe, Susannah Green
MetadataShow full item record
AbstractTermite gut flagellates are typically colonized by specific bacterial symbionts. Here we describe the phylogeny, ultrastructure and subcellular location of 'Candidatus Adiutrix intracellularis', an intracellular symbiont of Trichonympha collaris in the termite Zootermopsis nevadensis. It represents a novel, deep-branching clade of uncultured Deltaproteobacteria widely distributed in intestinal tracts of termites and cockroaches. Fluorescence in situ hybridization and transmission electron microscopy localized the endosymbiont near hydrogenosomes in the posterior part and near the ectosymbiont 'Candidatus Desulfovibrio trichonymphae' in the anterior part of the host cell. The draft genome of 'Ca. Adiutrix intracellularis' obtained from a metagenomic library revealed the presence of a complete gene set encoding the Wood-Ljungdahl pathway, including two homologs of fdhF encoding hydrogenase-linked formate dehydrogenases (FDHH ) and all other components of the recently described hydrogen-dependent carbon dioxide reductase (HDCR) complex, which substantiates previous claims that the symbiont is capable of reductive acetogenesis from CO2 and H2 . The close phylogenetic relationship between the HDCR components and their homologs in homoacetogenic Firmicutes and Spirochaetes suggests that the deltaproteobacterium acquired the capacity for homoacetogenesis via lateral gene transfer. The presence of genes for nitrogen fixation and the biosynthesis of amino acids and cofactors indicate the nutritional nature of the symbiosis.
Citation'Candidatus Adiutrix intracellularis', an endosymbiont of termite gut flagellates, is the first representative of a deep-branching clade of Deltaproteobacteria and a putative homoacetogen. 2016, 18 (8):2548-64 Environ. Microbiol.
AffiliationBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56, 38106 Braunschweig, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- 'Candidatus Ancillula trichonymphae', a novel lineage of endosymbiotic Actinobacteria in termite gut flagellates of the genus Trichonympha.
- Authors: Strassert JF, Köhler T, Wienemann TH, Ikeda-Ohtsubo W, Faivre N, Franckenberg S, Plarre R, Radek R, Brune A
- Issue date: 2012 Dec
- Genome of 'Ca. Desulfovibrio trichonymphae', an H(2)-oxidizing bacterium in a tripartite symbiotic system within a protist cell in the termite gut.
- Authors: Kuwahara H, Yuki M, Izawa K, Ohkuma M, Hongoh Y
- Issue date: 2017 Mar
- Candidatus Desulfovibrio trichonymphae, a novel intracellular symbiont of the flagellate Trichonympha agilis in termite gut.
- Authors: Sato T, Hongoh Y, Noda S, Hattori S, Ui S, Ohkuma M
- Issue date: 2009 Apr
- Parallel reductive genome evolution in Desulfovibrio ectosymbionts independently acquired by Trichonympha protists in the termite gut.
- Authors: Takeuchi M, Kuwahara H, Murakami T, Takahashi K, Kajitani R, Toyoda A, Itoh T, Ohkuma M, Hongoh Y
- Issue date: 2020 Sep
- Genome analysis of 'Candidatus Ancillula trichonymphae', first representative of a deep-branching clade of Bifidobacteriales, strengthens evidence for convergent evolution in flagellate endosymbionts.
- Authors: Strassert JFH, Mikaelyan A, Woyke T, Brune A
- Issue date: 2016 Oct