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dc.contributor.authorStichling, Nicole
dc.contributor.authorSuomalainen, Maarit
dc.contributor.authorFlatt, Justin W
dc.contributor.authorSchmid, Markus
dc.contributor.authorPacesa, Martin
dc.contributor.authorHemmi, Silvio
dc.contributor.authorJungraithmayr, Wolfgang
dc.contributor.authorMaler, Mareike D
dc.contributor.authorFreudenberg, Marina A
dc.contributor.authorPlückthun, Andreas
dc.contributor.authorMay, Tobias
dc.contributor.authorKöster, Mario
dc.contributor.authorFejer, György
dc.contributor.authorGreber, Urs F
dc.date.accessioned2018-03-23T15:32:45Z
dc.date.available2018-03-23T15:32:45Z
dc.date.issued2018-03
dc.identifier.citationLung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor. 2018, 14 (3):e1006914 PLoS Pathog.en
dc.identifier.issn1553-7374
dc.identifier.pmid29522575
dc.identifier.doi10.1371/journal.ppat.1006914
dc.identifier.urihttp://hdl.handle.net/10033/621333
dc.description.abstractMacrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleLung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen
refterms.dateFOA2018-06-13T14:13:27Z
html.description.abstractMacrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses.


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