Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor.
dc.contributor.author | Stichling, Nicole | |
dc.contributor.author | Suomalainen, Maarit | |
dc.contributor.author | Flatt, Justin W | |
dc.contributor.author | Schmid, Markus | |
dc.contributor.author | Pacesa, Martin | |
dc.contributor.author | Hemmi, Silvio | |
dc.contributor.author | Jungraithmayr, Wolfgang | |
dc.contributor.author | Maler, Mareike D | |
dc.contributor.author | Freudenberg, Marina A | |
dc.contributor.author | Plückthun, Andreas | |
dc.contributor.author | May, Tobias | |
dc.contributor.author | Köster, Mario | |
dc.contributor.author | Fejer, György | |
dc.contributor.author | Greber, Urs F | |
dc.date.accessioned | 2018-03-23T15:32:45Z | |
dc.date.available | 2018-03-23T15:32:45Z | |
dc.date.issued | 2018-03 | |
dc.identifier.citation | Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor. 2018, 14 (3):e1006914 PLoS Pathog. | en |
dc.identifier.issn | 1553-7374 | |
dc.identifier.pmid | 29522575 | |
dc.identifier.doi | 10.1371/journal.ppat.1006914 | |
dc.identifier.uri | http://hdl.handle.net/10033/621333 | |
dc.description.abstract | Macrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany. | en |
dc.identifier.journal | PLoS pathogens | en |
refterms.dateFOA | 2018-06-13T14:13:27Z | |
html.description.abstract | Macrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses. |