Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Parmar, Paresh A
Keane, Timothy J
Maynard, Stephanie A
Stevens, Molly M
MetadataShow full item record
AbstractExtracellular vesicles (EVs) have recently gained significant attention as important mediators of intercellular communication, potential drug carriers, and disease biomarkers. These natural cell-derived nanoparticles are postulated to be biocompatible, stable under physiological conditions, and to show reduced immunogenicity as compared to other synthetic nanoparticles. Although initial clinical trials are ongoing, the use of EVs for therapeutic applications may be limited due to undesired off-target activity and potential "dilution effects" upon systemic administration which may affect their ability to reach their target tissues. To fully exploit their therapeutic potential, EVs are embedded into implantable biomaterials designed to achieve local delivery of therapeutics taking advantage of enzyme prodrug therapy (EPT). In this first application of EVs for an EPT approach, EVs are used as smart carriers for stabilizing enzymes in a hydrogel for local controlled conversion of benign prodrugs to active antiinflammatory compounds. It is shown that the natural EVs' antiinflammatory potential is comparable or superior to synthetic carriers, in particular upon repeated long-term incubations and in different macrophage models of inflammation. Moreover, density-dependent color scanning electron microscopy imaging of EVs in a hydrogel is presented herein, an impactful tool for further understanding EVs in biological settings.
CitationEngineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy. 2018 Adv. Mater. Weinheim
AffiliationHIPS, Helmholtz-Institute für pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Extracellular vesicles: Novel promising delivery systems for therapy of brain diseases.
- Authors: Rufino-Ramos D, Albuquerque PR, Carmona V, Perfeito R, Nobre RJ, Pereira de Almeida L
- Issue date: 2017 Sep 28
- Extracellular vesicles for drug delivery.
- Authors: Vader P, Mol EA, Pasterkamp G, Schiffelers RM
- Issue date: 2016 Nov 15
- Achieving the Promise of Therapeutic Extracellular Vesicles: The Devil is in Details of Therapeutic Loading.
- Authors: Sutaria DS, Badawi M, Phelps MA, Schmittgen TD
- Issue date: 2017 May
- Engineered Extracellular Vesicles: Tailored-Made Nanomaterials for Medical Applications.
- Authors: Man K, Brunet MY, Jones MC, Cox SC
- Issue date: 2020 Sep 15
- Production of Extracellular Vesicles Loaded with Therapeutic Cargo.
- Authors: Lamichhane TN, Jay SM
- Issue date: 2018