Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsMzinza, David Twapokera
MetadataShow full item record
AbstractBronchus-associated lymphoid tissue (BALT) develops at unpredictable locations around lung bronchi following pulmonary inflammation. The formation and composition of BALT have primarily been investigated by immunohistology that, due to the size of the invested organ, is usually restricted to a limited number of histological sections. To assess the entire BALT of the lung, other approaches are urgently needed. Here, we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the lung. Using antibody staining of whole lung lobes and optical clearing by organic solvents, we present a method that allows in-depth visualization of the entire bronchial tree, the lymphatic vasculature and the immune cell composition of the induced BALT. Furthermore, three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced BALT. Using this approach, we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8% of the entire lung volume in mice deficient in CCR7, in contrast to wild type mice (WT). Furthermore, BALT induced by heat-inactivated E. coli is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice, in contrast to WT mice.Cellular and Molecular Immunology advance online publication, 12 February 2018; doi:10.1038/cmi.2017.150.
CitationApplication of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice. 2018 Cell. Mol. Immunol.
AffiliationTWINCORE, Zentrum für experimentelle uns klinische Ifektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
JournalCellular & molecular immunology
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
- Regulatory T cells interfere with the development of bronchus-associated lymphoid tissue.
- Authors: Kocks JR, Davalos-Misslitz AC, Hintzen G, Ohl L, Förster R
- Issue date: 2007 Apr 16
- Induction and Analysis of Bronchus-Associated Lymphoid Tissue.
- Authors: Fleige H, Förster R
- Issue date: 2017
- Subpopulations of lymphoid and non-lymphoid cells in bronchus-associated lymphoid tissue (BALT) of the mouse.
- Authors: Breel M, Van der Ende M, Sminia T, Kraal G
- Issue date: 1988 Apr
- The presence of specialized epithelial cells on the bronchus-associated lymphoid tissue (BALT) in the mouse.
- Authors: Tango M, Suzuki E, Gejyo F, Ushiki T
- Issue date: 2000 Mar
- IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
- Authors: Fleige H, Ravens S, Moschovakis GL, Bölter J, Willenzon S, Sutter G, Häussler S, Kalinke U, Prinz I, Förster R
- Issue date: 2014 Apr 7