Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture.
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Authors
Olmer, RuthEngels, Lena
Usman, Abdulai
Menke, Sandra
Malik, Muhammad Nasir Hayat
Pessler, Frank
Göhring, Gudrun
Bornhorst, Dorothee
Bolten, Svenja
Abdelilah-Seyfried, Salim
Scheper, Thomas
Kempf, Henning
Zweigerdt, Robert
Martin, Ulrich
Issue Date
2018-05-08
Metadata
Show full item recordAbstract
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.Citation
Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture. 2018, 10 (5):1657-1672 Stem Cell ReportsAffiliation
TWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.Journal
Stem cell reportsPubMed ID
29681541Type
ArticleLanguage
enISSN
2213-6711ae974a485f413a2113503eed53cd6c53
10.1016/j.stemcr.2018.03.017
Scopus Count
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- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
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