Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.
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Authors
Wang, XueZhou, Haibo
Chen, Hanna
Jing, Xiaoshu
Zheng, Wentao
Li, Ruijuan
Sun, Tao
Liu, Jiaqi
Fu, Jun
Huo, Liujie
Li, Yue-Zhong
Shen, Yuemao
Ding, Xiaoming
Müller, Rolf
Bian, Xiaoying
Zhang, Youming
Issue Date
2018-05-01
Metadata
Show full item recordAbstract
Bacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers. Precise genome engineering using bacteriophage recombinases is particularly useful for genome mining. However, recombinases are usually host-specific. The genome-guided discovery of novel recombinases and their transient expression could boost cryptic BGC mining. Herein, we reported a genetic system employing Red recombinases from Burkholderiales strain DSM 7029 for efficient genome engineering in several Burkholderiales species that currently lack effective genetic tools. Using specialized recombinases-assisted in situ insertion of functional promoters, we successfully mined five cryptic nonribosomal peptide synthetase/polyketide synthase BGCs, two of which were silent. Two classes of lipopeptides, glidopeptins and rhizomides, were identified through extensive spectroscopic characterization. This recombinase expression strategy offers utility within other bacteria species, allowing bioprospecting for potentially scalable discovery of novel metabolites with attractive bioactivities.Affiliation
HIPS, Helmholtz-Institute für pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.PubMed ID
29666226Type
ArticleThe following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
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