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dc.contributor.authorWheeler, Nicole E
dc.contributor.authorGardner, Paul P
dc.contributor.authorBarquist, Lars
dc.date.accessioned2018-06-18T13:59:04Z
dc.date.available2018-06-18T13:59:04Z
dc.date.issued2018-01-01
dc.identifier.issn1553-7404
dc.identifier.pmid29738521
dc.identifier.doi10.1371/journal.pgen.1007333
dc.identifier.urihttp://hdl.handle.net/10033/621404
dc.description.abstractEmerging pathogens are a major threat to public health, however understanding how pathogens adapt to new niches remains a challenge. New methods are urgently required to provide functional insights into pathogens from the massive genomic data sets now being generated from routine pathogen surveillance for epidemiological purposes. Here, we measure the burden of atypical mutations in protein coding genes across independently evolved Salmonella enterica lineages, and use these as input to train a random forest classifier to identify strains associated with extraintestinal disease. Members of the species fall along a continuum, from pathovars which cause gastrointestinal infection and low mortality, associated with a broad host-range, to those that cause invasive infection and high mortality, associated with a narrowed host range. Our random forest classifier learned to perfectly discriminate long-established gastrointestinal and invasive serovars of Salmonella. Additionally, it was able to discriminate recently emerged Salmonella Enteritidis and Typhimurium lineages associated with invasive disease in immunocompromised populations in sub-Saharan Africa, and within-host adaptation to invasive infection. We dissect the architecture of the model to identify the genes that were most informative of phenotype, revealing a common theme of degradation of metabolic pathways in extraintestinal lineages. This approach accurately identifies patterns of gene degradation and diversifying selection specific to invasive serovars that have been captured by more labour-intensive investigations, but can be readily scaled to larger analyses.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleMachine learning identifies signatures of host adaptation in the bacterial pathogen Salmonella enterica.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.en_US
refterms.dateFOA2018-06-18T13:59:04Z
dc.source.journaltitlePLoS genetics


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