Tracking gene expression and oxidative damage of O-stressed Clostridioides difficile by a multi-omics approach.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Metzendorf, Nicole G
Nuss, Aaron Mischa
Hofmann, Julia Danielle
MetadataShow full item record
AbstractClostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells with only few changes in the metabolite pattern. However, an extensive change in gene expression was determined by RNA-Seq. The most striking adaptation strategies involve a change in the reductive fermentation pathways of the amino acids proline, glycine and leucine. But also a far-reaching restructuring in the carbohydrate metabolism was detected with changes in the phosphotransferase system (PTS) facilitated uptake of sugars and a repression of enzymes of glycolysis and butyrate fermentation. Furthermore, a temporary induction in the synthesis of cofactor riboflavin was detected possibly due to an increased demand for flavin mononucleotid (FMN) and flavin adenine dinucleotide (FAD) in redox reactions. However, biosynthesis of the cofactors thiamin pyrophosphate and cobalamin were repressed deducing oxidation-prone enzymes and intermediates in these pathways. Micro-aerobically shocked cells were characterized by an increased demand for cysteine and a thiol redox proteomics approach revealed a dramatic increase in the oxidative state of cysteine in more than 800 peptides after 15 min of micro-aerobic shock. This provides not only a catalogue of oxidation-prone cysteine residues in the C. difficile proteome but also puts the amino acid cysteine into a key position in the oxidative stress response. Our study suggests that tolerance of C. difficile towards O
AffiliationHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
- Metabolic Reprogramming of <i>Clostridioides difficile</i> During the Stationary Phase With the Induction of Toxin Production.
- Authors: Hofmann JD, Otto A, Berges M, Biedendieck R, Michel AM, Becher D, Jahn D, Neumann-Schaal M
- Issue date: 2018
- Comprehensive Redox Profiling of the Thiol Proteome of <i>Clostridium difficile</i>.
- Authors: Sievers S, Dittmann S, Jordt T, Otto A, Hochgräfe F, Riedel K
- Issue date: 2018 May
- Transcriptomic profiling of Clostridium difficile grown under microaerophillic conditions.
- Authors: Giordano N, Hastie JL, Carlson PE
- Issue date: 2018 Mar 1
- The protein inventory of Clostridium difficile grown in complex and minimal medium.
- Authors: Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S
- Issue date: 2016 Oct
- Integration of metabolism and virulence in Clostridium difficile.
- Authors: Bouillaut L, Dubois T, Sonenshein AL, Dupuy B
- Issue date: 2015 May