CRP-cAMP mediates silencing of Salmonella virulence at the post-transcriptional level.
compendium figure 1 to 6 and ...
figures 1 to 6 and S11
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsEl Mouali, Youssef
Sánchez-Romero, María Antonia
Westermann, Alexander J
MetadataShow full item record
AbstractInvasion of epithelial cells by Salmonella enterica requires expression of genes located in the pathogenicity island I (SPI-1). The expression of SPI-1 genes is very tightly regulated and activated only under specific conditions. Most studies have focused on the regulatory pathways that induce SPI-1 expression. Here, we describe a new regulatory circuit involving CRP-cAMP, a widely established metabolic regulator, in silencing of SPI-1 genes under non-permissive conditions. In CRP-cAMP-deficient strains we detected a strong upregulation of SPI-1 genes in the mid-logarithmic growth phase. Genetic analyses revealed that CRP-cAMP modulates the level of HilD, the master regulator of Salmonella invasion. This regulation occurs at the post-transcriptional level and requires the presence of a newly identified regulatory motif within the hilD 3'UTR. We further demonstrate that in Salmonella the Hfq-dependent sRNA Spot 42 is under the transcriptional repression of CRP-cAMP and, when this transcriptional repression is relieved, Spot 42 exerts a positive effect on hilD expression. In vivo and in vitro assays indicate that Spot 42 targets, through its unstructured region III, the 3'UTR of the hilD transcript. Together, our results highlight the biological relevance of the hilD 3'UTR as a hub for post-transcriptional control of Salmonella invasion gene expression.
AffiliationHIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
- Small RNAs Activate Salmonella Pathogenicity Island 1 by Modulating mRNA Stability through the hilD mRNA 3' Untranslated Region.
- Authors: Abdulla SZ, Kim K, Azam MS, Golubeva YA, Cakar F, Slauch JM, Vanderpool CK
- Issue date: 2023 Jan 26
- A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA.
- Authors: López-Garrido J, Puerta-Fernández E, Casadesús J
- Issue date: 2014 May
- SlyA and HilD Counteract H-NS-Mediated Repression on the ssrAB Virulence Operon of Salmonella enterica Serovar Typhimurium and Thus Promote Its Activation by OmpR.
- Authors: Banda MM, Zavala-Alvarado C, Pérez-Morales D, Bustamante VH
- Issue date: 2019 Apr 15
- The transcriptional regulator SsrB is involved in a molecular switch controlling virulence lifestyles of Salmonella.
- Authors: Pérez-Morales D, Banda MM, Chau NYE, Salgado H, Martínez-Flores I, Ibarra JA, Ilyas B, Coombes BK, Bustamante VH
- Issue date: 2017 Jul
- Regulatory Evolution of the phoH Ancestral Gene in Salmonella enterica Serovar Typhimurium.
- Authors: Valdespino-Díaz MA, Rosales-Reyes R, De la Cruz MA, Bustamante VH
- Issue date: 2022 May 17