CRP-cAMP mediates silencing of Salmonella virulence at the post-transcriptional level.
Name:
compendium figure 1 to 6 and ...
Size:
612.0Kb
Format:
PDF
Description:
figures 1 to 6 and S11
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
El Mouali, YoussefGaviria-Cantin, Tania
Sánchez-Romero, María Antonia
Gibert, Marta
Westermann, Alexander J
Vogel, Jörg

Balsalobre, Carlos
Issue Date
2018-01-01
Metadata
Show full item recordAbstract
Invasion of epithelial cells by Salmonella enterica requires expression of genes located in the pathogenicity island I (SPI-1). The expression of SPI-1 genes is very tightly regulated and activated only under specific conditions. Most studies have focused on the regulatory pathways that induce SPI-1 expression. Here, we describe a new regulatory circuit involving CRP-cAMP, a widely established metabolic regulator, in silencing of SPI-1 genes under non-permissive conditions. In CRP-cAMP-deficient strains we detected a strong upregulation of SPI-1 genes in the mid-logarithmic growth phase. Genetic analyses revealed that CRP-cAMP modulates the level of HilD, the master regulator of Salmonella invasion. This regulation occurs at the post-transcriptional level and requires the presence of a newly identified regulatory motif within the hilD 3'UTR. We further demonstrate that in Salmonella the Hfq-dependent sRNA Spot 42 is under the transcriptional repression of CRP-cAMP and, when this transcriptional repression is relieved, Spot 42 exerts a positive effect on hilD expression. In vivo and in vitro assays indicate that Spot 42 targets, through its unstructured region III, the 3'UTR of the hilD transcript. Together, our results highlight the biological relevance of the hilD 3'UTR as a hub for post-transcriptional control of Salmonella invasion gene expression.Affiliation
HIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.PubMed ID
29879120Type
ArticleISSN
1553-7404ae974a485f413a2113503eed53cd6c53
10.1371/journal.pgen.1007401
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
Related articles
- A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA.
- Authors: López-Garrido J, Puerta-Fernández E, Casadesús J
- Issue date: 2014 May
- SlyA and HilD Counteract H-NS-Mediated Repression on the <i>ssrAB</i> Virulence Operon of <i>Salmonella enterica</i> Serovar Typhimurium and Thus Promote Its Activation by OmpR.
- Authors: Banda MM, Zavala-Alvarado C, Pérez-Morales D, Bustamante VH
- Issue date: 2019 Apr 15
- The transcriptional regulator SsrB is involved in a molecular switch controlling virulence lifestyles of Salmonella.
- Authors: Pérez-Morales D, Banda MM, Chau NYE, Salgado H, Martínez-Flores I, Ibarra JA, Ilyas B, Coombes BK, Bustamante VH
- Issue date: 2017 Jul
- Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium.
- Authors: Petrone BL, Stringer AM, Wade JT
- Issue date: 2014 Mar
- Integration of a complex regulatory cascade involving the SirA/BarA and Csr global regulatory systems that controls expression of the Salmonella SPI-1 and SPI-2 virulence regulons through HilD.
- Authors: Martínez LC, Yakhnin H, Camacho MI, Georgellis D, Babitzke P, Puente JL, Bustamante VH
- Issue date: 2011 Jun