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dc.contributor.authorStern, Christian
dc.contributor.authorKasnitz, Nadine
dc.contributor.authorKocijancic, Dino
dc.contributor.authorTrittel, Stephanie
dc.contributor.authorRiese, Peggy
dc.contributor.authorGuzman, Carlos A
dc.contributor.authorLeschner, Sara
dc.contributor.authorWeiss, Siegfried
dc.date.accessioned2018-07-25T09:40:30Z
dc.date.available2018-07-25T09:40:30Z
dc.date.issued2015-10-15
dc.identifier.issn1097-0215
dc.identifier.pmid25868911
dc.identifier.doi10.1002/ijc.29567
dc.identifier.urihttp://hdl.handle.net/10033/621431
dc.description.abstractFacultative anaerobic bacteria like E. coli can colonize solid tumors often resulting in tumor growth retardation or even clearance. Little mechanistic knowledge is available for this phenomenon which is however crucial for optimization and further implementation in the clinic. Here, we show that intravenous injections with E. coli TOP10 can induce clearance of CT26 tumors in BALB/c mice. Importantly, re-challenging mice which had cleared tumors showed that clearance was due to a specific immune reaction. Accordingly, lymphopenic mice never showed tumor clearance after infection. Depletion experiments revealed that during induction phase, CD8(+) T cells are the sole effectors responsible for tumor clearance while in the memory phase CD8(+) and CD4(+) T cells were involved. This was confirmed by adoptive transfer. CD4(+) and CD8(+) T cells could reject newly set tumors while CD8(+) T cells could even reject established tumors. Detailed analysis of adoptively transferred CD4(+) T cells during tumor challenge revealed expression of granzyme B, FasL, TNF-α and IFN-γ in such T cells that might be involved in the anti-tumor activity. Our findings should pave the way for further optimization steps of this promising therapy.en_US
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.29567en_US
dc.subjectCD4+en_US
dc.subjectCD8+en_US
dc.subjectE. coli Top10en_US
dc.subjectT cellsen_US
dc.subjecttumor antigen presentationen_US
dc.subjecttumor necrosisen_US
dc.titleInduction of CD4(+) and CD8(+) anti-tumor effector T cell responses by bacteria mediated tumor therapy.en_US
dc.typeArticleen_US
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en_US
refterms.dateFOA2020-10-06T19:41:02Z
dc.source.journaltitleInternational journal of cancer


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