Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors.

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Authors
Eicke, Dorothee
Baigger, Anja
Schulze, Kai cc
Latham, Sharissa L
Halloin, Caroline
Zweigerdt, Robert
Guzman, Carlos A
Blasczyk, Rainer
Figueiredo, Constança
Issue Date
2018-07-05

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Abstract
Megakaryocytes (MKs) are the precursors of platelets (PLTs) and may be used for PLT production in vivo or in vitro, as well as a source for PLT-derived growth factors. Induced pluripotent stem cells represent an unlimited cell source for the in vitro production of MKs. This study aimed at developing an effective, xeno-free and scalable system to produce high numbers of MKs. In particular, microcarrier beads-assisted stirred bioreactors were evaluated as a means of improving MK yields. This method resulted in the production of 18.7 × 10
Affiliation
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
URI
http://hdl.handle.net/10033/621437
DOI
10.1038/s41598-018-28459-x
PubMed ID
29977045
Type
Article
Language
en
ISSN
2045-2322
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States