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dc.contributor.authorHeidrich, Nadja
dc.contributor.authorHagmann, Antony
dc.contributor.authorBauriedl, Saskia
dc.contributor.authorVogel, Jörg
dc.contributor.authorSchoen, Christoph
dc.date.accessioned2018-08-28T13:54:37Z
dc.date.available2018-08-28T13:54:37Z
dc.date.issued2018-07-30
dc.identifier.issn1555-8584
dc.identifier.pmid30059276
dc.identifier.doi10.1080/15476286.2018.1486660
dc.identifier.urihttp://hdl.handle.net/10033/621456
dc.description.abstractNeisseria meningitidis, a commensal β-proteobacterium of the human nasopharynx, constitutes a worldwide leading cause of sepsis and epidemic meningitis. A recent genome-wide association study suggested an association of its type II-C CRISPR/Cas system with carriage and thus less invasive lineages. Here, we show that knock-out strains lacking the Cas9 protein are impaired in the adhesion to human nasopharyngeal cells which constitutes a central step in the pathogenesis of invasive meningococcal disease. Transcriptome sequencing data further suggest that meningococcal Cas9 does not affect the expression of surface adhesins but rather exerts its effect on cell adhesion in an indirect manner. Consequently, we speculate that the meningococcal CRISPR/Cas system exerts novel functions beyond its established role in defence against foreign DNA.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectCRISPR/Casen_US
dc.subjectCas9en_US
dc.subjectNeisseria meningitidisen_US
dc.subjectRIP-seqen_US
dc.subjectRNA-seqen_US
dc.subjectnasopharynxen_US
dc.subjectvirulenceen_US
dc.titleThe CRISPR/Cas system in Neisseria meningitidis affects bacterial adhesion to human nasopharyngeal epithelial cells.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.en_US
dc.source.journaltitleRNA biology


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