Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2018-08-02
Metadata
Show full item recordAbstract
Programmed -1 ribosomal frameshifting (-1PRF) is a recoding mechanism to make alternative proteins from a single mRNA transcript. -1PRF is stimulated by cis-acting signals in mRNA, a seven-nucleotide slippery sequence and a downstream secondary structure element, which is often a pseudoknot. In this study we engineered the frameshifting pseudoknot from the mouse mammary tumor virus to respond to a rationally designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate -1PRF both in vitro and in human cells. The results illustrate how NCTn-inducible -1PRF may serve as an important component of the synthetic biology toolbox for the precise control of gene expression using small synthetic molecules.Affiliation
HIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.PubMed ID
30085309Type
ArticleISSN
1362-4962ae974a485f413a2113503eed53cd6c53
10.1093/nar/gky689
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 United States
Related articles
- Structure, stability and function of RNA pseudoknots involved in stimulating ribosomal frameshifting.
- Authors: Giedroc DP, Theimer CA, Nixon PL
- Issue date: 2000 Apr 28
- Changed in translation: mRNA recoding by -1 programmed ribosomal frameshifting.
- Authors: Caliskan N, Peske F, Rodnina MV
- Issue date: 2015 May
- Structural and functional studies of retroviral RNA pseudoknots involved in ribosomal frameshifting: nucleotides at the junction of the two stems are important for efficient ribosomal frameshifting.
- Authors: Chen X, Chamorro M, Lee SI, Shen LX, Hines JV, Tinoco I Jr, Varmus HE
- Issue date: 1995 Feb 15
- Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting.
- Authors: Wang X, Xuan Y, Han Y, Ding X, Ye K, Yang F, Gao P, Goff SP, Gao G
- Issue date: 2019 Jan 24
- A mutant RNA pseudoknot that promotes ribosomal frameshifting in mouse mammary tumor virus.
- Authors: Kang H, Tinoco I Jr
- Issue date: 1997 May 15