Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.
dc.contributor.author | Matsumoto, Saki | |
dc.contributor.author | Caliskan, Neva | |
dc.contributor.author | Rodnina, Marina V | |
dc.contributor.author | Murata, Asako | |
dc.contributor.author | Nakatani, Kazuhiko | |
dc.date.accessioned | 2018-09-06T14:03:30Z | |
dc.date.available | 2018-09-06T14:03:30Z | |
dc.date.issued | 2018-08-02 | |
dc.identifier.issn | 1362-4962 | |
dc.identifier.pmid | 30085309 | |
dc.identifier.doi | 10.1093/nar/gky689 | |
dc.identifier.uri | http://hdl.handle.net/10033/621471 | |
dc.description.abstract | Programmed -1 ribosomal frameshifting (-1PRF) is a recoding mechanism to make alternative proteins from a single mRNA transcript. -1PRF is stimulated by cis-acting signals in mRNA, a seven-nucleotide slippery sequence and a downstream secondary structure element, which is often a pseudoknot. In this study we engineered the frameshifting pseudoknot from the mouse mammary tumor virus to respond to a rationally designed small molecule naphthyridine carbamate tetramer (NCTn). We demonstrate that NCTn can stabilize the pseudoknot structure in mRNA and activate -1PRF both in vitro and in human cells. The results illustrate how NCTn-inducible -1PRF may serve as an important component of the synthetic biology toolbox for the precise control of gene expression using small synthetic molecules. | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
dc.title | Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting. | en_US |
dc.type | Article | en_US |
dc.contributor.department | HIRI, Helmoltz-Institut für RNA-basierteInfektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany. | en_US |
refterms.dateFOA | 2018-09-06T14:03:30Z | |
dc.source.journaltitle | Nucleic acids research |