Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS.
Abstract
Fast (4100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow 1H RNA resonances and assign nucleotide spin systems with only 200 lg of uniformly 13C,15N-labelled RNA.Affiliation
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.PubMed ID
29974085Type
ArticleISSN
1364-548XScopus Count
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