Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro.
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Authors
Abeln, MarkusBorst, Kristina M
Cajic, Samanta
Thiesler, Hauke
Kats, Elina
Albers, Iris
Kuhn, Maike
Kaever, Volkhard
Rapp, Erdmann
Münster-Kühnel, Anja
Weinhold, Birgit
Issue Date
2017-07-04
Metadata
Show full item recordAbstract
The negatively charged nonulose sialic acid (Sia) is essential for murine development in vivo. In order to elucidate the impact of sialylation on differentiation processes in the absence of maternal influences, we generated mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia. Loss of CMAS activity resulted in an asialo cell surface accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues, as well as intracellular accumulation of free Sia. Remarkably, these changes did not impact intracellular metabolites or the morphology and transcriptome of pluripotent mESC lines. Moreover, the capacity of CmasAffiliation
TWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.PubMed ID
28374933Type
ArticleISSN
1439-7633ae974a485f413a2113503eed53cd6c53
10.1002/cbic.201700083
Scopus Count
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