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dc.contributor.authorAbeln, Markus
dc.contributor.authorBorst, Kristina M
dc.contributor.authorCajic, Samanta
dc.contributor.authorThiesler, Hauke
dc.contributor.authorKats, Elina
dc.contributor.authorAlbers, Iris
dc.contributor.authorKuhn, Maike
dc.contributor.authorKaever, Volkhard
dc.contributor.authorRapp, Erdmann
dc.contributor.authorMünster-Kühnel, Anja
dc.contributor.authorWeinhold, Birgit
dc.date.accessioned2018-10-12T08:16:40Z
dc.date.available2018-10-12T08:16:40Z
dc.date.issued2017-07-04
dc.identifier.issn1439-7633
dc.identifier.pmid28374933
dc.identifier.doi10.1002/cbic.201700083
dc.identifier.urihttp://hdl.handle.net/10033/621517
dc.description.abstractThe negatively charged nonulose sialic acid (Sia) is essential for murine development in vivo. In order to elucidate the impact of sialylation on differentiation processes in the absence of maternal influences, we generated mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia. Loss of CMAS activity resulted in an asialo cell surface accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues, as well as intracellular accumulation of free Sia. Remarkably, these changes did not impact intracellular metabolites or the morphology and transcriptome of pluripotent mESC lines. Moreover, the capacity of Cmasen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectCMP-sialic acid synthaseen_US
dc.subjectdifferentiationen_US
dc.subjectglycosylationen_US
dc.subjectmetabolismen_US
dc.subjectsialic acidsen_US
dc.titleSialylation Is Dispensable for Early Murine Embryonic Development in Vitro.en_US
dc.typeArticleen_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en_US
refterms.dateFOA2018-10-12T08:16:40Z
dc.source.journaltitleChembiochem : a European journal of chemical biology


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