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dc.contributor.authorMayer, Sabine
dc.contributor.authorMoeller, Rebecca
dc.contributor.authorMonteiro, João T
dc.contributor.authorEllrott, Kerstin
dc.contributor.authorJosenhans, Christine
dc.contributor.authorLepenies, Bernd
dc.date.accessioned2018-11-26T15:06:22Z
dc.date.available2018-11-26T15:06:22Z
dc.date.issued2018-01-01
dc.identifier.issn1664-3224
dc.identifier.pmid29487596
dc.identifier.doi10.3389/fimmu.2018.00213
dc.identifier.urihttp://hdl.handle.net/10033/621583
dc.description.abstractC-type lectin receptors (CLRs) are carbohydrate-binding receptors that recognize their ligands often in a Ca2+-dependent manner. Upon ligand binding, myeloid CLRs in innate immunity trigger or inhibit a variety of signaling pathways, thus initiating or modulating effector functions such as cytokine production, phagocytosis, and antigen presentation. CLRs bind to various pathogens, including viruses, fungi, parasites, and bacteria. The bacterium Campylobacter jejuni (C. jejuni) is a very frequent Gram-negative zoonotic pathogen of humans, causing severe intestinal symptoms. Interestingly, C. jejuni expresses several glycosylated surface structures, for example, the capsular polysaccharide (CPS), lipooligosaccharide (LOS), and envelope proteins. This “Methods” paper describes applications of CLR–Fc fusion proteins to screen for yet unknown CLR/bacteria interactions using C. jejuni as an example. ELISA-based detection of CLR/bacteria interactions allows a frst prescreening that is further confrmed by flow cytometry-based binding analysis and visualized using confocal microscopy. By applying these methods, we identifed Dectin-1 as a novel CLR recognizing two selected C. jejuni isolates with different LOS and CPS genotypes. In conclusion, the heredescribed applications of CLR–Fc fusion proteins represent useful methods to screen for and identify novel CLR/bacteria interactions.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectC-type lectin receptorsen_US
dc.subjectCampylobacter jejunien_US
dc.subjectDectin-1 receptoren_US
dc.subjectELISA assayen_US
dc.subjectconfocal microscopyen_US
dc.subjectflow cytometryen_US
dc.subjectinnate immunityen_US
dc.subjectscreening toolsen_US
dc.titleC-Type Lectin Receptor (CLR)-Fc Fusion Proteins As Tools to Screen for Novel CLR/Bacteria Interactions: An Exemplary Study on Preselected Isolates.en_US
dc.typeArticleen_US
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infektionsforschung GmbH,Feodor-Lynen Str. 7, 30625 Hannover, Germany.en_US
refterms.dateFOA2018-11-26T15:06:22Z
dc.source.journaltitleFrontiers in immunology


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