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dc.contributor.authorNolan, Laura M
dc.contributor.authorWhitchurch, Cynthia B
dc.contributor.authorBarquist, Lars
dc.contributor.authorKatrib, Marilyn
dc.contributor.authorBoinett, Christine J
dc.contributor.authorMayho, Matthew
dc.contributor.authorGoulding, David
dc.contributor.authorCharles, Ian G
dc.contributor.authorFilloux, Alain
dc.contributor.authorParkhill, Julian
dc.contributor.authorCain, Amy K
dc.date.accessioned2018-11-27T12:42:20Z
dc.date.available2018-11-27T12:42:20Z
dc.date.issued2018-11-01
dc.identifier.issn2057-5858
dc.identifier.pmid30383525
dc.identifier.doi10.1099/mgen.0.000229
dc.identifier.urihttp://hdl.handle.net/10033/621585
dc.description.abstractPseudomonas aeruginosa is an extremely successful pathogen able to cause both acute and chronic infections in a range of hosts, utilizing a diverse arsenal of cell-associated and secreted virulence factors. A major cell-associated virulence factor, the Type IV pilus (T4P), is required for epithelial cell adherence and mediates a form of surface translocation termed twitching motility, which is necessary to establish a mature biofilm and actively expand these biofilms. P. aeruginosa twitching motility-mediated biofilm expansion is a coordinated, multicellular behaviour, allowing cells to rapidly colonize surfaces, including implanted medical devices. Although at least 44 proteins are known to be involved in the biogenesis, assembly and regulation of the T4P, with additional regulatory components and pathways implicated, it is unclear how these components and pathways interact to control these processes. In the current study, we used a global genomics-based random-mutagenesis technique, transposon directed insertion-site sequencing (TraDIS), coupled with a physical segregation approach, to identify all genes implicated in twitching motility-mediated biofilm expansion in P. aeruginosa. Our approach allowed identification of both known and novel genes, providing new insight into the complex molecular network that regulates this process in P. aeruginosa. Additionally, our data suggest that the flagellum-associated gene products have a differential effect on twitching motility, based on whether components are intra- or extracellular. Overall the success of our TraDIS approach supports the use of this global genomic technique for investigating virulence genes in bacterial pathogens.en_US
dc.publisherMicrobiology Societyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleA global genomic approach uncovers novel components for twitching motility-mediated biofilm expansion in Pseudomonas aeruginosa.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
refterms.dateFOA2018-11-27T12:42:21Z
dc.source.journaltitleMicrobial genomics


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