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dc.contributor.authorPremnath, Priyanka
dc.contributor.authorReck, Michael
dc.contributor.authorWittstein, Kathrin
dc.contributor.authorStadler, Marc
dc.contributor.authorWagner-Döbler, Irene
dc.date.accessioned2018-12-11T09:53:43Z
dc.date.available2018-12-11T09:53:43Z
dc.date.issued2018-03-27
dc.identifier.issn1471-2180
dc.identifier.pmid29580208
dc.identifier.doi10.1186/s12866-018-1170-3
dc.identifier.urihttp://hdl.handle.net/10033/621613
dc.description.abstractWithin the polymicrobial dental plaque biofilm, bacteria kill competitors by excreting mixtures of bacteriocins, resulting in improved fitness and survival. Inhibiting their bacteriocin synthesis might therefore be a useful strategy to eliminate specific pathogens. We used Streptococcus mutans, a highly acidogenic inhabitant of dental plaque, as a model and searched for natural products that reduced mutacin synthesis. To this end we fused the promoter of mutacin VI to the GFP+ gene and integrated the construct into the genome of S. mutans UA159 by single homologous recombination. The resulting reporter strain 423p - gfp + was used to screen 297 secondary metabolites from different sources, mainly myxobacteria and fungi, for their ability to reduce the fluorescence of the fully induced reporter strain by > 50% while growth was almost unaffected (> 90% of control). Seven compounds with different chemical structures and different modes of action were identified. Erinacine C was subsequently validated and shown to inhibit transcription of all three mutacins of S. mutans. The areas of the inhibition zones of the sensor strains S. sanguinis and Lactococcus lactis were reduced by 35% to 61% in comparison to controls in the presence of erinacine C, demonstrating that the amount of active mutacins in the culture supernatants of S. mutans was reduced. Erinacines are cyathane diterpenes that were extracted from cultures of the edible mushroom Hericium erinaceus. They have anti-inflammatory, antimicrobial and neuroprotective effects. For erinacine C, a new biological activity was found here. We demonstrate the successful development of a whole-cell fluorescent reporter for the screening of natural compounds and report that erinacine C suppresses mutacin synthesis in S. mutans without affecting cell viability.en_US
dc.publisherBMCen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectFluorescent reporteren_US
dc.subjectFungien_US
dc.subjectMutacinen_US
dc.subjectMyxobacteriaen_US
dc.subjectSecondary metabolitesen_US
dc.subjectStreptococcusen_US
dc.subjectVirulenceen_US
dc.subjectWhole-cell screeningen_US
dc.titleScreening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
refterms.dateFOA2018-12-11T09:53:44Z
dc.source.journaltitleBMC microbiology


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