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dc.contributor.authorLorenz, Anne
dc.contributor.authorPreuße, Matthias
dc.contributor.authorBruchmann, Sebastian
dc.contributor.authorPawar, Vinay
dc.contributor.authorGrahl, Nora
dc.contributor.authorPils, Marina C
dc.contributor.authorNolan, Laura M
dc.contributor.authorFilloux, Alain
dc.contributor.authorWeiss, Siegfried
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2018-12-13T09:50:30Z
dc.date.available2018-12-13T09:50:30Z
dc.date.issued2018-11-08
dc.identifier.issn1462-2920
dc.identifier.pmid30411474
dc.identifier.doi10.1111/1462-2920.14468
dc.identifier.urihttp://hdl.handle.net/10033/621615
dc.description.abstractPseudomonas aeruginosa is an environmental microorganism and a causative agent of diverse acute and chronic, biofilm-associated infections. Advancing research-based knowledge on its adaptation to conditions within the human host is bound to reveal novel strategies and targets for therapeutic intervention. Here, we investigated the traits that P. aeruginosa PA14 as well as a virulence attenuated ΔlasR mutant need to survive in selected murine infection models. Experimentally, the genetic programs that the bacteria use to adapt to biofilm-associated versus acute infections were dissected by passaging transposon mutant libraries through mouse lungs (acute) or mouse tumours (biofilm-infection). Adaptive metabolic changes of P. aeruginosa were generally required during both infection processes. Counter-selection against flagella expression was observed during acute lung infections. Obviously, avoidance of flagella-mediated activation of host immunity is advantageous for the wildtype bacteria. For the ΔlasR mutant, loss of flagella did not confer a selective advantage. Apparently, other pathogenesis mechanisms are active in this virulence attenuated strain. In contrast, the infective process of P. aeruginosa in the chronic biofilm model apparently required expression of flagellin. Together, our findings imply that the host immune reactions against the infectious agent are very decisive for acuteness and duration of the infectious disease. They direct disease outcome.en_US
dc.publisherWiley-Blackwellen_US
dc.relationinfo:eu-repo/grantAgreement/ERC/H2020/ 724290en_US
dc.rightsembargoedAccessen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleImportance of flagella in acute and chronic Pseudomonas aeruginosa infections.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.source.journaltitleEnvironmental microbiology


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