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dc.contributor.authorKingsley, Robert A
dc.contributor.authorLangridge, Gemma
dc.contributor.authorSmith, Sarah E
dc.contributor.authorMakendi, Carine
dc.contributor.authorFookes, Maria
dc.contributor.authorWileman, Tom M
dc.contributor.authorEl Ghany, Moataz Abd
dc.contributor.authorKeith Turner, A
dc.contributor.authorDyson, Zoe A
dc.contributor.authorSridhar, Sushmita
dc.contributor.authorPickard, Derek
dc.contributor.authorKay, Sally
dc.contributor.authorFeasey, Nicholas
dc.contributor.authorWong, Vanessa
dc.contributor.authorBarquist, Lars
dc.contributor.authorDougan, Gordon
dc.date.accessioned2018-12-19T11:44:21Z
dc.date.available2018-12-19T11:44:21Z
dc.date.issued2018-11-18
dc.identifier.issn1462-2920
dc.identifier.pmid30450829
dc.identifier.doi10.1111/1462-2920.14458
dc.identifier.urihttp://hdl.handle.net/10033/621620
dc.description.abstractContaminated water is a major risk factor associated with the transmission of Salmonella enterica serovar Typhi (S. Typhi), the aetiological agent of human typhoid. However, little is known about how this pathogen adapts to living in the aqueous environment. We used transcriptome analysis (RNA-seq) and transposon mutagenesis (TraDIS) to characterize these adaptive changes and identify multiple genes that contribute to survival. Over half of the genes in the S. Typhi genome altered expression level within the first 24 h following transfer from broth culture to water, although relatively few did so in the first 30 min. Genes linked to central metabolism, stress associated with arrested proton motive force and respiratory chain factors changed expression levels. Additionally, motility and chemotaxis genes increased expression, consistent with a scavenging lifestyle. The viaB-associated gene tviC encoding a glcNAc epimerase that is required for Vi polysaccharide biosynthesis was, along with several other genes, shown to contribute to survival in water. Thus, we define regulatory adaptation operating in S. Typhi that facilitates survival in water.en_US
dc.publisherWiley-Blackwellen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleFunctional analysis of Salmonella Typhi adaptation to survival in water.en_US
dc.typeArticleen_US
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
refterms.dateFOA2018-12-19T11:44:22Z
dc.source.journaltitleEnvironmental microbiology


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