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dc.contributor.authorThanikkal, Edvin J
dc.contributor.authorGahlot, Dharmender K
dc.contributor.authorLiu, Junfa
dc.contributor.authorFredriksson Sundbom, Marcus
dc.contributor.authorGurung, Jyoti M
dc.contributor.authorRuuth, Kristina
dc.contributor.authorFrancis, Monika K
dc.contributor.authorObi, Ikenna R
dc.contributor.authorThompson, Karl M
dc.contributor.authorChen, Shiyun
dc.contributor.authorDersch, Petra
dc.contributor.authorFrancis, Matthew S
dc.date.accessioned2019-01-16T14:12:39Z
dc.date.available2019-01-16T14:12:39Z
dc.date.issued2018-12-06
dc.identifier.issn2150-5608
dc.identifier.pmid30518290
dc.identifier.doi10.1080/21505594.2018.1556151
dc.identifier.urihttp://hdl.handle.net/10033/621655
dc.description.abstractThe Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Concomitantly, this pathway also down-regulates key determinants of virulence. In Yersinia, cpxA deletion accumulates high levels of phosphorylated CpxR (CpxR~P). Accumulated CpxR~P directly repressed rovA expression and this limited expression of virulence-associated processes. A second transcriptional regulator, RovM, also negatively regulates rovA expression in response to nutrient stress. Hence, this study aimed to determine if CpxR~P can influence rovA expression through control of RovM levels. We determined that the active CpxR~P isoform bound to the promoter of rovM and directly induced its expression, which naturally associated with a concurrent reduction in rovA expression. Site-directed mutagenesis of the CpxR~P binding sequence in the rovM promoter region desensitised rovM expression to CpxR~P. These data suggest that accumulated CpxR~P inversely manipulates the levels of two global transcriptional regulators, RovA and RovM, and this would be expected to have considerable influence on Yersinia pathophysiology and metabolism.en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectEnvironmental stress responsivenessen_US
dc.subjectfitnessen_US
dc.subjectgene expression controlen_US
dc.subjectgrowth and survivalen_US
dc.subjectmetabolic networksen_US
dc.subjectmicrobial behaviouren_US
dc.titleThe Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
refterms.dateFOA2019-01-16T14:12:41Z
dc.source.journaltitleVirulence


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