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dc.contributor.authorRose, Rebekah
dc.contributor.authorHäuser, Svenja
dc.contributor.authorStump-Guthier, Carolin
dc.contributor.authorWeiss, Christel
dc.contributor.authorRohde, M
dc.contributor.authorKim, Kwang Sik
dc.contributor.authorIshikawa, Hiroshi
dc.contributor.authorSchroten, Horst
dc.contributor.authorSchwerk, Christian
dc.contributor.authorAdam, Rüdiger
dc.date.accessioned2019-01-17T15:00:19Z
dc.date.available2019-01-17T15:00:19Z
dc.date.issued2018-11-21
dc.identifier.issn1574-6968
dc.identifier.pmid30476042
dc.identifier.doi10.1093/femsle/fny274
dc.identifier.urihttp://hdl.handle.net/10033/621658
dc.description.abstractEscherichia coli is the most common Gram-negative causative agent of neonatal meningitis and E. coli meningitis is associated with high morbidity and mortality. Previous research has been carried out with regard to the blood-brain barrier and thereby unveiled an assortment of virulence factors involved in E. coli meningitis. Little, however, is known about the role of the blood-cerebrospinal fluid (CSF) barrier (BCSFB), in spite of several studies suggesting that the choroid plexus (CP) is a possible entry point for E. coli into the CSF spaces. Here, we used a human CP papilloma (HIBCPP) cell line that was previously established as valid model for the study of the BCSFB. We show that E. coli invades HIBCPP cells in a polar fashion preferentially from the physiologically relevant basolateral side. Moreover, we demonstrate that deletion of outer membrane protein A, ibeA or neuDB genes results in decreased cell infection, while absence of fimH enhances invasion, although causing reduced adhesion to the apical side of HIBCPP cells. Our findings suggest that the BCSFB might constitute an entry point for E. coli into the central nervous system, and HIBCPP cells are a valuable tool for investigating E. coli entry of the BCSFB.en_US
dc.language.isoenen_US
dc.publisherOxford Academic Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleVirulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro.en_US
dc.typeArticleen_US
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFEMS microbiology lettersen_US
dc.source.journaltitleFEMS microbiology letters


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