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dc.contributor.authorCastoldi, Arianna
dc.contributor.authorEmpting, Martin
dc.contributor.authorDe Rossi, Chiara
dc.contributor.authorMayr, Karsten
dc.contributor.authorDersch, Petra
dc.contributor.authorHartmann, Rolf
dc.contributor.authorMüller, Rolf
dc.contributor.authorGordon, Sarah
dc.contributor.authorLehr, Claus-Michael
dc.date.accessioned2019-01-18T15:45:31Z
dc.date.available2019-01-18T15:45:31Z
dc.date.issued2018-12-05
dc.identifier.citationPharm Res. 2018 Dec 5;36(1):22. doi: 10.1007/s11095-018-2521-3.en_US
dc.identifier.issn1573-904X
dc.identifier.pmid30519925
dc.identifier.doi10.1007/s11095-018-2521-3
dc.identifier.urihttp://hdl.handle.net/10033/621663
dc.description.abstractThe objective of this work was to evaluate the potential of polymeric spherical and aspherical invasive nanocarriers, loaded with antibiotic, to access and treat intracellular bacterial infections. Aspherical nanocarriers were prepared by stretching of spherical precursors, and both aspherical and spherical nanocarriers were surface-functionalized with the invasive protein InvA497. The relative uptake of nanocarriers into HEp-2 epithelial cells was then assessed. Nanocarriers were subsequently loaded with a preparation of the non-permeable antibiotic gentamicin, and tested for their ability to treat HEp-2 cells infected with the enteroinvasive bacterium Shigella flexneri. InvA497-functionalized nanocarriers of both spherical and aspherical shape showed a significantly improved rate and extent of uptake into HEp-2 cells in comparison to non-functionalized nanocarriers. Functionalized and antibiotic-loaded nanocarriers demonstrated a dose dependent killing of intracellular S. flexneri. A slight but significant enhancement of intracellular bacterial killing was also observed with aspherical as compared to spherical functionalized nanocarriers at the highest tested concentration. InvA497-functionalized, polymer-based nanocarriers were able to efficiently deliver a non-permeable antibiotic across host cell membranes to affect killing of intracellular bacteria. Functionalized nanocarriers with an aspherical shape showed an interesting future potential for intracellular infection therapy.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAOT-gentamicinen_US
dc.subjectaspherical nanoparticlesen_US
dc.subjectbacteriomimetic nanocarriersen_US
dc.subjectintracellular infectionen_US
dc.subjectinvasinen_US
dc.titleAspherical and Spherical InvA497-Functionalized Nanocarriers for Intracellular Delivery of Anti-Infective Agents.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalPharmaceutical researchen_US
refterms.dateFOA2019-01-18T15:45:32Z
dc.source.journaltitlePharmaceutical research


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